Description
Engineering of high-performance chromatography medium, has long been esteemed as an art rather than science, this raised a great challenge in securing separation precision, method transferability and scaling-up in purification of biomolecules. Herein, we report a bottom-up engineering strategy for chromatography medium based on 3D printing. Combining micrometer stereolithography and nanometer pore chemistry, the strategy enables precision engineering of high-performance separation medium in diverse scale, shape and chemistry. Between 1,000 devices, extreme performance consistency was demonstrated by the superb column-to-column and batch-to-batch reproducibility (retention time CV 2.04%). Ultra-fast and high-resolution separations of intact proteins and monoclonal antibodies were realized in reversed-phase and hydrophobic interaction chromatography: within 1 minute, resolution > 1.5 was achieved. Purification process of native proteins was directly amplified three orders of magnitude from μg to mg: 12 mg native hemeproteins were isolated within 8 min at negligible scaling-up cost, supporting a liter-scale processing capacity within 7 h on one 20 mm i.d. column. With unprecedented advantages in method and material prototyping, fully automatic and parallel production capacity, high-fidelity microstructure and chemistry across dimensions, and highly efficient method transfer and scaling-up, the stereolithography engineered chromatography medium may open a green and sustainable path to speeding up separation and purification processes in analytical, pilot-scale and mass-production of biomolecular entities, as demanded in biosynthesis and pharmaceutical industries.| Period | 22 Oct 2024 |
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| Event title | 53rd International Symposium on High Performance Liquid Phase Separations and Related Techniques |
| Event type | Conference |
| Location | Dalian, China |
| Degree of Recognition | International |