Human onchocerciasis is a Neglected Tropical Disease (NTD) which results in severe itching, skin lesions and diverse degrees of vision impairment. The disease is caused by the parasite Onchocerca volvulus and transmitted to humans by repeated bites of infective black flies of the genus Simulium. The enormity of its public health and socio-economic burden stimulated the creation of various large-scale control programs that have relied on Mass Drug Administration (MDA) of ivermectin in endemic communities for the elimination of this disease. The impact of these control measures has been substantial and there has been a successful interruption of transmission in most parts of the Americas. Parasitological evaluations of the disease in endemic regions of Cameroon have shown significant progress in parasite transmission interruption in some regions. However, the ‘Guidelines for Stopping MDA and Verifying Elimination of Human Onchocerciasis’ by WHO strongly recommend the detection of O. volvulus DNA in black flies via O-150 PCR poolscreen. Notwithstanding, in the context of Cameroon and Africa at large, poolscreen testing has been largely neglected making it very hard to know if, and when, to stop MDA. Thus, this project aims to detect the presence of O. volvulus DNA in pools of black flies using O-150 PCR assay in onchocerciasis endemic areas of Cameroon.