DescriptionThe secondment was divided in two parts. The first part was the attendance of the meeting "New Frontiers" on LSECs biology and nanoscopy in Sommaroy Norway. Multiple researchers in the field of LSECs/vessel biology, tissue engineering & microfluidics and nanoscopy presented their work and I presented my own work on LSECs in chronic liver disease. The second part of the stay was at the research group Vascular Biology at the Artic University of Norway were I prepared multicellular liver spheroids for transmission (TEM) and scanning electron microscopy (SEM). The aim of this stay was to investigate if LSECs maintain their morphology hence still have fenestrae when cultured with other cells types in our multicellular spheroids. During the stay I was trained for the preparation of samples for TEM/SEM and was trained to work with the TEM microscope. Numerous spheroids cultured for 4 or 7 days were prepared and imaged with SEM and TEM. SEM showed multiple LSECs with fenestrae and sieve plates (clustered fenestrae) which demonstrated the maintenance of LSEC morphology in our multicellular liver spheroids. In addition, other structures were found such as microvilli and bile cuniculi indicating the maintenance of hepatocyte morphology in our spheroid model.
|Period||25 Nov 2021 → 3 Dec 2021|
|Held at||Vascular Biology research group - Artic University of Norway (UiT)|
|Degree of Recognition||International|