Macroscale fluorescence lifetime imaging with novel time-gated camera complemented with high-resolution color image registration

Sahakian, S. (Speaker)
Janssen, S. (Contributor)
Van Den Dries, T. (Contributor)
Lapauw, T. (Contributor)
Marcus Stroet (Contributor)
Lepoutte, T. (Contributor)
Iranian, P. (Contributor)
Kuijk, M. (Contributor)
Hernot, S. (Contributor)
Ingelberts, H. (Contributor)

Activity: Talk or presentation › Talk or presentation at a conference

Description

Macroscale fluorescence lifetime (FLT) imaging is emerging as a promising tool to improve tumor margin delineation and enhance contrast between tumor and healthy tissue during fluorescence-guided surgery. We have been developing the tauCAM, a custom-built time-gated CMOS camera with a high quantum efficiency (QE) in NIR (46%) and a resolution of 128x128 pixels, specifically for this application. The images obtained from the tauCAM are overlaid on high-resolution color images acquired with a dedicated color camera. We demonstrate the capabilities of our camera by performing in vivo studies on subcutaneous mice tumor models administered with nanobody- and antibody-based NIR tumor-targeted fluorescent contrast agents. The results indicate that FLT imaging enhances the contrast between tumor and healthy tissue.

Period	7 Apr 2024
Event title	SPIE Photonics Europe 2024
Event type	Conference
Location	Strasbourg, France
Degree of Recognition	International