Proof-of-concept of fluorescence lifetime endoscopy using a novel nanosecond time-gated CAPS camera

Activity: Talk or presentationTalk or presentation at a conference

Description

Fluorescence imaging has long been employed in various fields like microscopy, and (pre)clinical imaging. However, there is growing interest in harnessing the temporal behavior of fluorophores, known as fluorescence lifetime, to gain deeper insights into the properties of a sample.
Our research department is currently working on the development of a new imaging technology. This camera, based on the Current-Assisted Photonic Sampler (CAPS), aims to enable real-time fluorescence lifetime imaging, particularly in the NIR (700-900nm) region, and holds the potential to improve the precision of tumor removal in image-guided surgeries.
Conventional endoscopy is based on full-spectrum white light. However, utilizing a NIR fluorescence-based endoscopy system and incorporating fluorescence lifetime, supplemented by white light, would provide additional information to distinguish between malignant and benign tissues, where the naked eye cannot distinguish them.
This work focuses on the potential of the CAPS camera for real-time fluorescence lifetime imaging with the latest iteration housing a new 64 x 64-pixel sensor and rigid endoscope. We perform experiments on different fluorescence phantoms and process the data utilizing two distinct lifetime calculation methods: the classical time-domain curve fitting and the frequency-domain phasor approach.
The capabilities of the system are demonstrated in an experimental setup where we illuminate the sample with a picosecond pulsed laser. Due to the independence of fluorescence lifetime to the intensity and uniformity of the excitation light, we can observe a uniform lifetime image even when the intensity image is not.

Period27 Jan 2024
Event titleSPIE Photonics West 2024
Event typeConference
LocationSan Fransisco, United States, California
Degree of RecognitionInternational