Crystal structure of the 5-phosphatase domain of Synaptojanin1 in complex with a nanobody



Synaptojanin1 (Synj1) is a phosphoinositide phosphatase, important in clathrin uncoating during endocytosis of presynaptic vesicles. It was identified as a potential drug target for Alzheimer's disease, Down syndrome, and TBC1D24-associated epilepsy, while also loss-of-function mutations in Synj1 are associated with epilepsy and Parkinson's disease. Despite its involvement in a range of disorders, structural, and detailed mechanistic information regarding the enzyme is lacking. Here, we report the crystal structure of the 5-phosphatase domain of Synj1. Moreover, we also present a structure of this domain bound to the substrate diC8-PI(3,4,5)P 3 , providing the first image of a 5-phosphatase with a trapped substrate in its active site. Together with an analysis of the contribution of the different inositide phosphate groups to catalysis, these structures provide new insights in the Synj1 mechanism. Finally, we analysed the effect of three clinical missense mutations (Y793C, R800C, Y849C) on catalysis, unveiling the molecular mechanisms underlying Synj1-associated disease.
Date made available30 Dec 2020
PublisherProtein Data Bank in Europe

Flemish discipline codes

  • Structural biology


  • Crystal structure
  • Parkinson's disease
  • epilepsy
  • inositol polyphosphate 5-phosphatase
  • phosphoinositide
  • nanobody


  • Format
  • mmCIF
  • pdb
  • pdf
  • fasta
  • mtz

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