Karine Breckpot

2025

Cancer vaccines based on mRNA: Hype or hope?

de Mey, W., Autaers, D., Bertazzon, G., Esprit, A., Marco Aragon, M., Franceschini, L. & Breckpot, K., 6 Feb 2025, Trends in mRNA Vaccine Research. Wiley VCH, p. 337-400 64 p.

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Research › peer-review

Open Access

Efficient α and β- radionuclide therapy targeting fibroblast activation protein-α in an aggressive preclinical mouse tumour model

Ceuppens, H., Pombo Antunes, A. R., Navarro, L., Ertveldt, T., Berdal, M., Nagachinta, S., De Ridder, K., Lahoutte, T., Keyaerts, M., Devoogdt, N., Goyvaerts, C., D'Huyvetter, M. & Breckpot, K., Jan 2025, In: European Journal of Nuclear Medicine and Molecular Imaging. 52, 2, p. 444-457 14 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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3 Citations (Scopus)

5 Downloads (Pure)

2024

Alpha-galactosylceramide improves the potency of mRNA LNP vaccines against cancer and intracellular bacteria

Meulewaeter, S., Aernout, I., Deprez, J., Engelen, Y., De Velder, M., Franceschini, L., Breckpot, K., Van Calenbergh, S., Asselman, C., Boucher, K., Impens, F., De Smedt, S. C., Verbeke, R. & Lentacker, I., Jun 2024, In: Journal of Controlled Release. 370, p. 379-391 13 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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6 Citations (Scopus)

18 Downloads (Pure)

Anti-Idiotypic VHHs and VHH-CAR-T Cells to Tackle Multiple Myeloma: Different Applications Call for Different Antigen-Binding Moieties

Hanssens, H., Meeus, F., Gesquiere, E. L., Puttemans, J., Vlaeminck, Y. D., Veirman, K. D., Breckpot, K. & Devoogdt, N., 22 May 2024, In: International Journal of Molecular Sciences. 25, 11, 16 p., 5634.

Research output: Contribution to journal › Article › peer-review

Open Access

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2 Citations (Scopus)

10 Downloads (Pure)

Assessment of connexin43 hemichannel functionality based on cytosolic uptake of Yo-Pro1

Van Campenhout, R., Sanz-Serrano, J., Cooreman, A., De Vlaeminck, Y., Breckpot, K., Kadam, P., Tabernilla Garcia, A. & Vinken, M., 6 Apr 2024, In: Methods in Molecular Biology. 2801, p. 75-85 11 p., 6.

Research output: Contribution to journal › Article

Open Access

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3 Downloads (Pure)

Assessment of connexin43 hemichannel functionality based on cytosolic uptake of Yo-Pro1

Van Campenhout, R., Sanz-Serrano, J., Cooreman, A., De Vlaeminck, Y., Breckpot, K., Kadam, P., Tabernilla Garcia, A. & Vinken, M., 2024, Assessment of connexin43 hemichannel functionality based on cytosolic uptake of Yo-Pro1. Mammano, F. & Retamal, M. (eds.). Methods in Molecular Biology, Vol. 2801. p. 75-85

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Research

AXL as immune regulator and therapeutic target in Acute Myeloid Leukemia: from current progress to novel strategies

Vandewalle, N., De Beule, N., De Becker, A., De Bruyne, E., Menu, E., Vanderkerken, K., Breckpot, K., Devoogdt, N. & De Veirman, K., Dec 2024, In: Experimental Hematology and Oncology. 13, 1, 20 p., 99.

Research output: Contribution to journal › Scientific review › peer-review

Open Access

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9 Downloads (Pure)

AXL-specific single domain antibodies show diagnostic potential and anti-tumor activity in Acute Myeloid Leukemia

Vandewalle, N., Satilmis, H., Verheye, E., Fan, R., Wang, Y., De Groof, T., Bridoux, J., kerre, T., De Beule, N., De Becker, A., De Bruyne, E., Menu, E., Vanderkerken, K., Breckpot, K., Devoogdt, N. & De Veirman, K., 15 Apr 2024, In: Theranostics. 14, 7, p. 2656-2674 19 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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5 Citations (Scopus)

11 Downloads (Pure)

Cap-to-Tail Analysis of HLA-I antigen encoding mRNA

Esprit, A., Autaers, D., Breckpot, K. & Franceschini, L., 24 Jan 2024, (Unpublished).

Research output: Unpublished contribution to conference › Poster

Development and evaluation of a single domain antibody targeting folate receptor alpha for radioligand therapy

Dewulf, J., Massa, S., Navarro, L., Dekempeneer, Y., Santens, F., Ceuppens, H., Breckpot, K., Van Ginderachter, J. A., Lahoutte, T., D'Huyvetter, M. & Devoogdt, N., 19 Dec 2024, In: Journal of Nanobiotechnology. 22, 1, 13 p., 763.

Research output: Contribution to journal › Article › peer-review

Open Access

1 Citation (Scopus)

Fueling CARs: metabolic strategies to enhance CAR T-cell therapy

Van der Vreken, A., Vanderkerken, K., De Bruyne, E., De Veirman, K., Breckpot, K. & Menu, E., 10 Jul 2024, In: Experimental Hematology and Oncology. 13, 1, 17 p., 66.

Research output: Contribution to journal › Article › peer-review

Open Access

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4 Citations (Scopus)

9 Downloads (Pure)

Generation and Characterization of Novel Pan-Cancer Anti-uPAR Fluorescent Nanobodies as Tools for Image-Guided Surgery

Mateusiak, Ł., Floru, S., De Groof, T. W. M., Wouters, J., Declerck, N. B., Debie, P., Janssen, S., Zeven, K., Breckpot, K., Puttemans, J., Vincke, C., Devoogdt, N. & Hernot, S., Aug 2024, In: Advanced Science. 11, 30, 11 p., e2400700.

Research output: Contribution to journal › Article › peer-review

Open Access

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3 Citations (Scopus)

14 Downloads (Pure)

Generic semi-automated radiofluorination strategy for single domain antibodies: [18F]FB-labelled single domain antibodies for PET imaging of fibroblast activation protein-α or folate receptor-α overexpression in cancer

Dierick, H., Navarro, L., Ceuppens, H., Ertveldt, T., Pombo Antunes, A. R., Keyaerts, M., Devoogdt, N., Breckpot, K., D'Huyvetter, M., Lahoutte, T., Caveliers, V. & Bridoux, J., Dec 2024, In: EJNMMI Radiopharmacy and Chemistry. 9, 1, 14 p., 54.

Research output: Contribution to journal › Article › peer-review

Open Access

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3 Citations (Scopus)

18 Downloads (Pure)

Noninvasive nuclear imaging of the immune checkpoint TIGIT using different nanobody-scaffolds and radionuclides

Zeven, K., Dierick, H., Sevenois, M., Baudhuin, H. V., Bridoux, J., Navarro, L., De Groof, T., Raes, G., Breckpot, K. & Devoogdt, N., 13 Mar 2024, (Unpublished).

Research output: Unpublished contribution to conference › Poster

Novel technologies for applying immune checkpoint blockers

Awad, R. M. & Breckpot, K., 18 Jan 2024, International Review of Cell and Molecular Biology. Elsevier Inc., 100 p. (International Review of Cell and Molecular Biology).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Research › peer-review

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2 Citations (Scopus)

62 Downloads (Pure)

Optimizing high-intensity focused ultrasound-induced immunogenic cell-death using passive cavitation mapping as a monitoring tool

Engelen, Y., Krysko, D. V., Effimova, I., Breckpot, K., Versluis, M., De Smedt, S., Lajoinie, G. & Lentacker, I., Nov 2024, In: Journal of Controlled Release. 375, p. 389-403 15 p.

Research output: Contribution to journal › Article › peer-review

1 Downloads (Pure)

Preclinical evaluation of antigen-sensitive B7-H3-targeting nanobody-based CAR-T cells in glioblastoma cautions for on-target, off-tumor toxicity

Meeus, F., Funeh, C. N., Awad, R. M., Zeven, K., Autaers, D., De Becker, A., Van Riet, I., Goyvaerts, C., Tuyaerts, S., Neyns, B., Devoogdt, N., De Vlaeminck, Y. & Breckpot, K., 19 Nov 2024, In: Journal for ImmunoTherapy of Cancer. 12, 11, 16 p., e009110.

Research output: Contribution to journal › Article › peer-review

Open Access

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1 Citation (Scopus)

6 Downloads (Pure)

Scrutiny of chimeric antigen receptor activation by the extracellular domain: experience with single domain antibodies targeting multiple myeloma cells highlights the need for case-by-case optimization

Hanssens, H., Meeus, F., De Vlaeminck, Y., Lecocq, Q., Puttemans, J., Debie, P., De Groof, T. W. M., Goyvaerts, C., De Veirman, K., Breckpot, K. & Devoogdt, N., 2024, In: Frontiers in Immunology. 15, 15 p., 1389018.

Research output: Contribution to journal › Article › peer-review

Open Access

Sustained release of a human pd-l1 single-domain antibody using peptide-based hydrogels

Heremans, J., Maximilian Awad, R., Bridoux, J., Ertveldt, T., Caveliers, V., Madder, A., Hoogenboom, R., Devoogdt, N., Ballet, S., Hernot, S., Breckpot, K. & Martin, C., Mar 2024, In: European Journal of Pharmaceutics and Biopharmaceutics. 196, 196, 21 p., 114183.

Research output: Contribution to journal › Article › peer-review

Open Access

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5 Citations (Scopus)

153 Downloads (Pure)

The development of Nanobodies against the next-generation immune checkpoint TIGIT with diagnostic and therapeutic potential

Zeven, K., De Groof, T., Raes, G., Breckpot, K. & Devoogdt, N., 18 Apr 2024, (Unpublished).

Research output: Unpublished contribution to conference › Poster

2023

Accepted poster session at ImmunoRad 2023: Targeted alpha therapy using Actinium-225 radiolabeled single domain antibodies induces antigen-specific immune responses and instills immunomodulation both systemically and at the tumor microenvironment

Ertveldt, T., Krasniqi, A., Ceuppens, H., Puttemans, J., Dekempeneer, Y., De Jonghe, K., de Mey, W., Lecocq, Q., De Vlaeminck, Y., Awad, R. M., Goyvaerts, C., De Veirman, K., Morgenstern, A., Bruchertseifer, F., Keyaerts, M., Devoogdt, N., D'Huyvetter, M. & Breckpot, K., 27 Sep 2023.

Research output: Unpublished contribution to conference › Poster

An mRNA mix redirects dendritic cells towards an antiviral program, inducing anticancer cytotoxic stem cell and central memory CD8+ T cells

de Mey, W., Locy, H., De Ridder, K., De Schrijver, P., Autaers, D., Lakdimi, A., Esprit, A., Franceschini, L., Thielemans, K., Verdonck, M. & Breckpot, K., 4 Mar 2023, In: Frontiers in Immunology. 14, 10 p., 1111523.

Research output: Contribution to journal › Article › peer-review

Open Access

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8 Citations (Scopus)

49 Downloads (Pure)

Assessment of Human SARS CoV-2-Specific T-Cell Responses Elicited In Vitro by New Computationally Designed mRNA Immunogens: COVARNA

Esteban, I., Pastor-Quinones, C., Usero, L., Aurrecoechea, E., Franceschini, L., Esprit, A., Gelpi, J. L., Martinez-Jimenez, F., Lopez-Biga, N., Breckpot, K., Thielemans, K., Leal, L., Gomez, C. E., Sistere-Oro, M., Meyerhans, A., Esteban, M., Alonso, M. J., Garcia, F. & Plana, M., 22 Dec 2023, In: Vaccines. 12, 1, p. 15-30 16 p., 15.

Research output: Contribution to journal › Article › peer-review

Open Access

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22 Downloads (Pure)

Challenges in neoantigen-directed therapeutics

Lybaert, L., Lefever, S., Fant, B., Smits, E., De Geest, B., Breckpot, K., Dirix, L., Feldman, S. A., van Criekinge, W., Thielemans, K., van der Burg, S. H., Ott, P. A. & Bogaert, C., 9 Jan 2023, In: Cancer Cell. 41, 1, p. 15-40 26 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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65 Citations (Scopus)

127 Downloads (Pure)

Colorectal Cancer Immunotherapy: State of the Art and Future Directions

Cornista, A. M., Giolito, M. V., Baker, K., Hazime, H., Dufait, I., Datta, J., Khumukcham, S. S., De Ridder, M., Roper, J., Abreu, M. T., Breckpot, K. & Van Der Jeught, K., Jan 2023, In: Gastro Hep Advances. 2, 8, p. 1103-1119 17 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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13 Citations (Scopus)

160 Downloads (Pure)

Open Access

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9 Citations (Scopus)

45 Downloads (Pure)

Elevated LAG-3 expression in plasma cells and lymphoid subpopulations at distinct stages of Multiple Myeloma disease progression

Satilmis, H., Vandewalle, N., Verheye, E., Fan, R., De Bruyne, E., Menu, E., Devoogdt, N., Vanderkerken, K., Breckpot, K. & De Veirman, K., 29 Sep 2023, (Unpublished).

Research output: Unpublished contribution to conference › Poster

Gompertz Model Use in Tumor Growth Curves Analysis

Olarte, O., Ertveldt, T., Meulewaeter, S., Keyaerts, M., Dewitte, H., Verbeke, R., Lentacker, I., Breckpot, K. & Barbé, K., 7 Mar 2023, Mathematical Biosciences: Elsevier, 31 p.

Research output: Working paper › Preprint

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8 Downloads (Pure)

Intraoperative tumor visualization through a specific fluorescently-labeled anti-uPAR targeting single-domain antibody

Vangeloven, E., Mateusiak, L., Floru, S., De Groof, T., Declerck, N., Wouters, J., Janssen, S., Debie, P., Zeven, K., Vincke, C., Puttemans, J., Stroet, M., Ballet, S., Breckpot, K., Devoogdt, N. & Hernot, S., 2023, (Unpublished).

Research output: Unpublished contribution to conference › Poster

In vitro modelling of local gene therapy with IL-15/IL-15Rα and a PD-L1 antagonist in melanoma reveals an interplay between NK cells and CD4+ T cells

Awad, R. M., De Vlaeminck, Y., Meeus, F., Ertveldt, T., Zeven, K., Ceuppens, H., Goyvaerts, C., Verdonck, M., Salguero, G., Raes, G., Devoogdt, N. & Breckpot, K., Dec 2023, In: Scientific reports. 13, 1, p. 1-13 13 p., 18995.

Research output: Contribution to journal › Article › peer-review

Open Access

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3 Citations (Scopus)

17 Downloads (Pure)

In vivo optical imaging of glioma using a novel uPAR-targeting fluorescent single-domain antibody

Mateusiak, L., Floru, S. (ed.), De Groof, T. (ed.), Declerck, N. (ed.), Wouters, J. (ed.), Janssen, S. (ed.), Debie, P. (ed.), Zeven, K. (ed.), Vincke, C. (ed.), Puttemans, J. (ed.), Stroet, M. (ed.), Breckpot, K. (ed.), Devoogdt, N. (ed.) & Hernot, S. (ed.), 17 Mar 2023.

Research output: Unpublished contribution to conference › Unpublished abstract

In vivo optical imaging using a novel fluorescently-labeled single-domain antibody targeting a universal tumor marker

Mateusiak, L., Floru, S., Wouters, J., De Groof, T., Declerck, N., Janssen, S., Debie, P., Zeven, K., Puttemans, J., Breckpot, K., Devoogdt, N. & Hernot, S., 28 Jan 2023.

Research output: Unpublished contribution to conference › Unpublished abstract

Nanobody-mediated SPECT/CT imaging reveals the spatiotemporal expression of programmed death-ligand 1 in response to a CD8+ T cell and iNKT cell activating mRNA vaccine

Ertveldt, T., Meulewaeter, S., De Vlaeminck, Y., Olarte, O., Broos, K., Van Calenbergh, S., Bourgeois, S., Deprez, J., Heremans, Y., Goyvaerts, C., Staels, W., De Smedt, S., Dewitte, H., Devoogdt, N., Keyaerts, M., Verbeke, R., Barbé, K., Lentacker, I. & Breckpot, K., 9 Oct 2023, In: Theranostics. 13, 15, p. 5483-5500 18 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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7 Citations (Scopus)

57 Downloads (Pure)

Phase I Study of [68Ga]Ga-Anti-CD206-sdAb for PET/CT Assessment of Protumorigenic Macrophage Presence in Solid Tumors (MMR Phase I)

Gondry, O., Xavier, C., Raes, L., Heemskerk, J., Devoogdt, N., Everaert, H., Breckpot, K., Lecocq, Q., Decoster, L., Fontaine, C., Schallier, D., Aspeslagh, S., Vaneycken, I., Raes, G., Van Ginderachter, J. A., Lahoutte, T., Caveliers, V. & Keyaerts, M., Sep 2023, In: Journal of Nuclear Medicine. 64, 9, p. 1378-1384 7 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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24 Citations (Scopus)

50 Downloads (Pure)

Recent advances using genetic therapies against infectious diseases and for vaccination

Galy, A., Berkhout, B., Breckpot, K., Pichon, C., Bloom, K., Kiem, H-P., Mühlebach, M. D. & McCune, J. M., Sep 2023, In: Human Gene Therapy. 34, 17-18, p. 896-904 9 p.

Research output: Contribution to journal › Article › peer-review

Open Access

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