2D electrophoretic analysis of protein expression in salivarygland myoepithelial cells.

Project Details

Description

Myoepithelial cells in salivary glands have not been the subject of much research in the past. Based on preliminary results, their protein expression pattern shows concordances with stellate cells in other organs. In order to assess the protein expression pattern in physiological and pathological conditions myoepithelial cells from rat salivary glands will be purified and cultured. Supernatant and homogenates from these cultures will be subjected to a 2D gelelectrophoretic separation and analyzed according to the proteomics approach. The pathological models applied are the duct ligation method and caerulein injection. Salicary glands from control and experimental animals are the duct ligation method and caerulein injection. Salivary glands from control and experimental animals are explanted, enzymatically digested and sorted (FACSaria). 2D gel electrophoresisis performed on the homogenates from control and experimental cells thus obtained. This will be done using a different fluorochrome for each cell group in order to obtain a maximal standardization of the procedure. Differences in protein expression of both cell groups can thus be analyzed in one procedure. A parallel grant application has been introduced at FWO, the present application encompasses 2*12000 EUR for working expenses and an investment for a colour-sensitive gel scanner.
AcronymOZR1529
StatusFinished
Effective start/end date1/01/0731/12/08

Flemish discipline codes

  • Basic sciences

Keywords

  • dentistry