Analysis of trypanosomosis-associated destruction of immunological memory during T. evansi infections

Project Details

Description

Trypanosomes cause disease in a wide host range, including humans, livestock and game animals. Historically, trypanosomosis has been an "African" disease, but parasites that no longer need tseste fly transmission have now found their way into South America, Asia and Europe. T. evansi is in this respect the most successful and widespread trypanosome, causing a world-wide infection issue. For some time now, it is known that T. evansi infections cause secondary problems including the inhibition of various existing livestock vaccines.
Our laboratory has been investigating trypanosomosis-associated B-cell dyfunction for several years, and we have described in detail the phenomenon of infection-associated destruction of the host B- cell compartment. So far we have not unraveled the issues that undermine immunological memory and vaccine recall responses, as tools to do so have been limited in the past. With new cellular markers, and a proposal to produce a recombinant fluorescent trypanosome molecule that can be used as a cellular tag for FACS analysis, we envisage here to analyse in detail the problems that
occur in T. evansi infections. Our focus will go specifically to the genetic analysis of Marginal Zone B- cells dysfunction (the IgM antibody compartment is crucial to control the initial wave of parasitemia during the onset of infection) and memory B-cells crucial during an immunological recall response. Also, a published vaccine protocol will be functionally scrutinized.
Short titleAnalysis of trypanosomosis
AcronymFWOAL894
StatusActive
Effective start/end date1/01/1831/12/21

Keywords

  • trypanosomosis
  • destruction of immunological memory
  • evansi infections

Flemish discipline codes

  • Biomedical modelling