Design and prototyping of microfluidic chip technology for spatial three-dimensional liquid chromatography

Project Details


Spatial three-dimensional liquid chromatography (3D-LC) entails a novel chromatography concept which allows for unprecedented high-resolution LC separations. Separations will be performed by making analytes migrate to different positions in a three-dimensional separation body. The maximum achievable separation performance is the product of the peak capacities realized in each separation stage. Analyte fractions are analyzed in parallel, which greatly reduces analysis time.
This project aims at the design and construction of microfluidic chip technology for spatial 3D-LC. High-resolution 3D printing will be applied to prototype microfluidic devices with interconnected channel structures, based on a Pareto-optimality approach. Novel flow-control principles will be explored, employing pressure- and electro-driven elutions and using solvent mixing/demixing approaches. Nested monolithic nanostructures will be synthesized in-situ in the microchannels allowing for orthogonal separations. Detection of analytes will be realized by interfacing of a spatial separation devise with detection methods after a “printing technique”, i.e. immobilizing the effluent from the final separation on a suitable substrate at regular intervals. In addition, a novel multi-nozzle ESI interface allowing for sample storage will be developed. A proof-of-concept study will be conducted to demonstrate the separation of a complex proteinaceous sample containing 1,000,000 components in an overnight analysis.
Effective start/end date1/01/1831/12/21

Flemish discipline codes

  • Microfluidics/flow chemistry


  • microfluidics