Determination of the genome sequence of the acetic acid bacterium Acetobacter pasteurianus 386B as starting point to unravel the functional role of acetic acid bacteria in the cocoa bean fermentation process

Project Details

Description

This project aims at determining one of the first genome sequences in the genus Acetobacter, more precisely of A. pasteurianus 386B, an own isolate obtained from a spontaneous cocoa bean heap fermentation process in Ghana. Hereto, a combination of two sequencing methods will be used: an innovative method developed by 454 Life Sciences/Roche, based on pyrosequencing, and the classical Sanger sequencing method. The ultimate aim is to elucidate the functionality of AAB in complex ecosystems by means of genomics and to develop microarrays to study gene expression. The genome sequence will be the basis for annotation by means of comparative analysis using publicly available sequence data, and by applying gene prediction algorithms. This way, the genetic potential of this bacteria can be studied, which is important in eventual applications in industrial processes. Using this information, the metabolism can be mapped and specific pathways can be revealed, among which mechanisms that give A. pasteurianus its competitive advantage in the spontaneous cocoa bean heap fermentation processes. Further, substrate usage can be predicted, as well as the potential to exhibit negative traits, such as antibiotic resistance and the production of virulence factors. The protein-coding genes will be the basis to develop in-silico oligonucleotides, that will be used to compose the first AAB microarray after a thorough validation. This microarray will be used to study gene expression of AAB in complex ecosystems.
AcronymFWOKN212
StatusFinished
Effective start/end date1/01/0931/12/09

Flemish discipline codes

  • Biological sciences
  • Chemical sciences
  • Materials engineering

Keywords

  • Molecular Biotechnology
  • Applied Biology