Development of an extremely-high pressure liquid chromatography instrument for gradient elution separation of complex environmental and biological samples

Project Details


A leap in separation performance is required to push the limits of current liquid phase chromatographic analytical separation systems towards the extremely-high efficiencies required to analyze the highly complex environmental and biological samples that are being presented today. The most straightforward method to improve chromatographic performance is an increase in maximum operating pressure. Whereas current commercial instrumentation is limited to pressure a little over 1000bar,an in-house built system is currently developed in our lab that can operate at extreme pressures up to 2600bar. The current set-up however only allows to work with a very limited number of solvents and a constant mobile phase composition. Under these conditions,it is not possible to analyze the wide variety of complex sample for which the system is envisioned. The goal of this research project is therefore to develop an automated system that can deliver a wide variety of solvents,solvent mixtures and buffers in both an isocratic (constant) and gradient (varying composition) mode. Besides the construction of the device and its application for the analysis of the complex samples,the fundamental effects of the use of these extremely high pressures on e.g. phase equilibrium and physicochemical properties will be investigated. In addition, the dissipation of the viscous forces working in the packed bed chromatographic columns,leading to a significant release of heat, will be taken under consideration.
Effective start/end date1/01/1531/12/15

Flemish discipline codes

  • Biological sciences


  • Applied Biology