Diagnosis of genetic disease in preimplantation embryos.

Improve the technology for the detection of genetic disease in human embryos from couples carrying a disease mutation. Develop a primer extension preamplification (PEP) system that can be used in preimplantation diagnosis. Investigate the possibilities of PCR amplification of trinucleotide repeats from a single blastomere for diagnosis of Huntington disease, myotonic dystrophy and fragile X syndrome.