Fast chromatographic methods on new stationary phases

    Project Details

    Description

    Two subjects will be examined.
    The first subject concerns the evaluation of fast separations on monolithic silica columns.
    Method transfer, robustness and column ageing are examined by means of several case studies. Robustness testing is done for the methods, for which the transfer is successful. Then, the analysis time is reduced by increasing the flow rate and the robustness of the separations is evaluated. Column ageing was studied by repeated injections of a test mixture during the use of the column.

    The second part concerns the determination of the antioxidant capacity of green tea extracts (or more general of a global sample property) from chromatograms. The aim is to find a model that relates a standard colorimetric method to predict the total antioxidant capacity of the extracts with a fast HPLC method. The advantage of an HPLC method is that it includes a separation and allows an estimation of the amount of each polyphenol individually. To reduce the analysis times, monolithic silica columns will be used and incomplete resolved chromatograms can be used. Chemometric pre-processing method (e.g. Correlation Optimized Warping) will be used to align all chromatograms and Partial Least Squares regression to model
    AcronymFWOTM270
    StatusFinished
    Effective start/end date1/10/0130/09/05

    Keywords

    • correlation optimized warping
    • monolithic columns
    • deconvolution
    • fast HPLC
    • antioxidant capacity

    Flemish discipline codes

    • Mathematical sciences
    • Chemical sciences
    • Pharmaceutical sciences