Heterogeneity, differentiation and kinetic of multiple myeloma cells: study of the 5T2 MM in the C57BLKaLwRij mouse

Project Details

Description

Multiple myeloma (MM) is mainly localized in the bone marrow both in humans and the 5T2 MM mouse model. This intrinsic property of the MM cells lays on the basis of this project. The underlying mechanisms of the specific homing to the bone marrow are unknown. Two hypothesizes are proposed: firstly the specific localization might be a result of a selective transmigration through the bone marrow endothelium while in the second hypothesis the bone marrow microenvironment may provide unique survival and growth stimuli for the MM cells. A combination of both hypothesizes is also possible. These hypothesizes will be tested in the 5T2 MM mouse model. This model shows all the characteristics of the clinical situation of humans. The 5T2 MM cells isolated appear to be heterogeneous. The aim of the project is to sort these populations, enabling us to identify which of population is responsible for the homing and proliferation of the MM cells after intravenous transfer. Subsequently we will try to reveal the characteristics of this clonogenic population. We will study the proliferative capacity, the localization in different organs, expression of antigens and transendothelial migration through bone marrow endothelial cells. The results of these studies should enable us to continue with the cell population responsible for the transendothelial migration through bone marrow endothelial cells and proliferation in the extravascular compartment of the bone marrow.
AcronymPRIBI13
StatusFinished
Effective start/end date1/10/9731/12/99

Keywords

  • immunology
  • multiple myeloma
  • oncology

Flemish discipline codes

  • Basic sciences