Impact of the new molecular-virological technique such as cell-free synthesis and quantitative PCR on the study of the replication cycle of poliovirus.

  • Rombaut, Bartholomeus, (Administrative Promotor)
  • Vrijsen, Raphael, (Co-Promotor)

Project Details

Description

Poliovirus is without any discussion a well studied virus. Although a lot of work was done by different groups, some aspects of the replication cycle are still not elucidated. This study remains interesting, not only from a fundamental point of view, but it might result in applications, such as finding specific targets for antiviral chemotherapy.Goal of this project is to boost our work on the uncoating and the morphogenesis of poliovirus, by the introduction of innovative techniques, such as cell-free synthesis and quantitative PCR.We possess now a cell-free system in which, after addition of viral RNA, infectious virus is synthesised. The system is now being further developed for the study of the morphogenesis of poliovirus.On the other hand, we are now developing a PCR technique for the quantitation of virus in samples of different origin (IWT project). One of the goals of this project is to get insight in the uncoating mechanism of poliovirus.
AcronymOZR51
StatusFinished
Effective start/end date1/01/9731/12/01

Keywords

  • morphogenesis
  • virus replication
  • poliovirus
  • uncoating
  • PCR

Flemish discipline codes

  • Basic sciences
  • Biological sciences
  • Pharmaceutical sciences