Mapping out organellar redox signaling networks in plants

  • Messens, Joris (Administrative Promotor)
  • Nguyen, Ho Thuy Dung, (Mandate)

Project Details

Description

To survive various environmental stress conditions, plants rely on signal transduction events, and
one of these signals are reduction-oxidation (redox) signals. How these redox signals are perceived,
triggered and transduced is still not fully understood. To define redox communication, we will use
the plant model system Chlamydomonas reinhardtii. Firstly, we will follow how the levels of the
redox metabolite H2O2 changes in organellar cross-talk experiments. As a rapid first redox
response, H2O2 reacts with cysteine thiols on proteins, forming sulfenylated cysteines. Secondly,
we will trap and identify these cysteine-modified proteins in the organelles where we observed an
increase of H2O2. Thirdly, we aim to study the oxidation kinetics and the induced structural
changes on one of the redox-sensing proteins from a curated list of organellar proteins.
With this approach, we will get new perspectives on organellar H2O2 communication in plants and
on the pathways that might be manipulated to help plants cope with environmental variations.
Short title or EU acronymOZR backup mandate
AcronymOZR3347
StatusFinished
Effective start/end date1/01/1931/12/19

Keywords

  • redox signaling
  • organellar communication
  • structural and functional profiling
  • molecular genetics
  • biochemistry plants