Micellar liquis chromatography, an alternative to immobilized artificial membranes when screening for potential drugs.

  • Massart, Desire, (Administrative Promotor)

    Project Details

    Description

    When screening for drug candidates it is essential to know their possibilty of passing biological membranes, because their pharmacological effect depends upon it. The in - vitro models of the intestinal membranes, although good predictors, do not allow high-throughput screening. Therefore attention is paid to chromatographic methods. Classical RP-HPLC systems have been investigated as to determine through to be a parameter representative of the main transport mechanism over a bio-membrane. A recent development is the chromatographic technique using immobilized artificial membrane (IAM) columns. It seems that these stationary phase models of membranes can be regarded as even better screeners of the passing of the bio-membranes. Because there are some technical problems with this phase, micellar liquid chromatography (MLC) which also has more interfacial properties seems to be an alternative/ The aim is to make MLC operational as an alternative for the IAM methodology when trying to predict the biological activity of candidate dugs and to evaluate it in a quantitative structure retention relationship (QSRR)context.
    AcronymIWT97
    StatusFinished
    Effective start/end date1/10/9930/09/03

    Keywords

    • IAM
    • bio-membrane passage
    • MLC

    Flemish discipline codes

    • (Bio)chemical engineering
    • Pharmaceutical sciences
    • Chemical sciences