Nanobodies as crystallization chaperones for challenging biomedical targets

Project Details

Description

In the early stages of structural genomics, challenging targets such as those that are poorly soluble, unstable, intrinsically (partially) unstructured, or are highly flexible have been put aside. In the last years, we proved the principle that Nanobodies can be used as crystallization chaperones in the crystallization and structure determination of challenging target proteins that would prove unsolvable using more conventional strategies. In the frame of the current project, we want to exploit these unique properties of Nanobodies to facilitate the production, the purification, the crystallization and the functional characterization of a number of protein families that present particular experimental challenges. For each of these families, we can build on a particular expertise or technology platform, giving us a competitive advantage or a unique approach in these fields.
AcronymFWOAL551
StatusFinished
Effective start/end date1/01/1031/12/13

Flemish discipline codes

  • Biological sciences

Keywords

  • Applied Biology