This study aims a better knowledge about the peptide neurotransmitter Neuropeptide Y (NPY) and its receptors. These receptors comprise at least 3 subtypes (Y1, Y2, Y3) and non-peptide antagonists are of medical interest because of their potental hypotensive a appetite-reducing properties. A) Comparison of the binding of labelled NPY with the binding of recently developed non-peptide antagonists on e.g. cell lines of human origin (Y1: SK-N-MC, Y2: SK-N-BE). B) Human Y1-receptors are in their original "surrounding" in the SK-N-MC cells but not in transfected CHO cells. We wish to study the consequence of this at the level of receptor-mediated persponses: intracellular cyclic AMP and calcium concentrations and the release of acid metabolites. C) Determination of the density of NPY receptor subtypes in membrane preparations and thin sections from different regions of the human brain under normal and pathological conditions to provide furthrer information concerning the functional role of NPY in the CNS. D) We recently discovered a conotoxin which binds NPY with nanomolar affinity. We plan to complete purification and structural characterisation of this peptide, as well as to compare its NPY-binding properties with those of anti-NPY antibodies and NPY receptor subtypes.
|Effective start/end date||1/01/98 → 22/04/99|
Flemish discipline codes
- Biological sciences