Nieuw financieringsmodel onderzoek: ZAP-startkrediet 2012

  • Gutierrez Gonzalez, Gustavo, (Administrative Promotor)
  • Snyder, Evan (Collaborator)

Project Details

Description

Function and Role of the APC/C in neural stem cells differentiation

Stem cells are capable of faithfully proliferate in saecula saeculorum while preserving their infinite potential to become (differentiate into) any particular cell type of our body. For this reason, stem cells represent a virtually unlimited source of functional human cells for basic research and regenerative medicine technologies. Understanding the factors responsible of their differentiation into neurons will for example be crucial to the development of novel therapies towards a vast array of neurological diseases (such as Parkinson's, Alzheimer's, or Multiple Sclerosis). However, molecular mechanisms involved in stem cells neuronal differentiation remain largely unknown, especially processes coupling environmental cues to exit of the cell proliferation cycle before differentiation.

We propose to understand how a major component of the cell division machinery (the ubiquitin ligase APC/C-Cdh1) is regulated in stem cells during their differentiation into neurons. In addition, we will characterize how the APC/C-Cdh1 promotes exit from the cell cycle and subsequent neuronal differentiation of stem cells. Our studies will use human embryonic stem cells (hESCs) but will largely focus on human induced pluripotent stem cells (hIPSCs). Recent advances have allowed the conversion of hIPSCs; cells which become sufficiently dedifferentiated (reprogrammed) that they now emulate hESCs and following specific differentiation protocols, are capable to also differentiate into neurons. Molecular characterization of the stem cells differentiation process would allow the design of tools capable of modulating stem cells properties and potentialities with therapeutic objectives.
AcronymOZR2339
StatusFinished
Effective start/end date1/03/121/03/16

Flemish discipline codes

  • Morphological sciences
  • Oncology
  • Systems biology
  • Molecular and cell biology

Keywords

  • stem cells
  • neuronal differentiation
  • proteasome
  • cell cycle
  • phosphorylation
  • ubiquitin