Optimization of fertility preservation after gonadotoxic therapies

Project Details

Description

The spermatogonial stem cell transplantation (SSCT) and testicular tissue graft are two promising techniques for the prevention of infertility after gonadotoxic therapies. Further research on the optimization and efficiency are needed before the step to clinical application can be taken. In the first part of the study, the efficiency of the xenogeneic tissue transplantation with human prepubertal tissue will be optimized. A human prepubertal testicular fragment will be transplanted into the testis of an immune deficient mouse. Four and nine months after transplantation testicular tissue will be collected and evaluated. Spermatogonial stem cell survival and functionality and maturation of the niche cells will be evaluated. Human recombinant follicle stimulating hormone will be administered to investigate whether survival, proliferation and differentiation can be induced in human spermatogonia, grafted in the murine testicular environment. In a second part of the study, we will investigate whether apoptotic mechanisms can influence the efficiency of SSCT or grafting. At various time points after transplantation (SSCT and graft) apoptosis will be studied through early and late apoptotic markers. In the third part, we will investigate the role of anti-apoptotic agents on apoptosis and the efficiency of transplantation.
AcronymFWOKN219
StatusFinished
Effective start/end date1/01/1031/12/10

Flemish discipline codes

  • Basic sciences
  • Biological sciences

Keywords

  • reproductive genetics
  • andrology
  • clinical genetics
  • embryology
  • assisted reproductive technology