Quantitavive analysis of SC lipids after tape stripping combined with non-invasive efficacy measurements.

Project Details


The barrier function of the skin is situated in the stratum corneum and consists of horny keratinocytes surrounded by lamellar lipid bilayers, and a hydrolipidic film present on the skin's surface. Some skin pathologies show an impaired barrier, but it is not sufficiently known which lipids are involved, nor at what level the changes occur.
By developping a standardised non-invasive 'stripping' model, direct measurements on volunteers' skin will be possible by bioengineering techniques. The barrierfunction of the skin will be measured by TEWL, hydration by capacitance measurements, skin colour by chromametry, microrelief of the skin using a light transmission method and pH by potentiometric pH measurements. The quantitative and qualitative lipid profile of the taken strip will be analysed in function of the SC depth, using chromatografic-fotodensitometric scanning techniques. Parameters influencing the inter- and intraindividual lipid profiles, such as gender, age, location on the body, phototype and experimental damages (SLS- or aceton treatments) will be studied.
After the characterisation of the healthy skin, the barrier function of pathological skin (atopic skin) will be investigated and efficient topic products will be developed for the treatment and normalisation.
Effective start/end date1/10/01 → 31/12/03


  • Diagnosis (barrier function)
  • Cosmetologie
  • Dermatologie
  • Physiology
  • toxicology
  • Dermatology
  • Fysiologie
  • Diagnose (barrièrefunctie)
  • Cosmetology

Flemish discipline codes

  • Electrical and electronic engineering
  • Basic sciences