Strategies for neuroprotection in models for global and focal cerebral ischaemia in the rat.

Project Details

Description

An animal model for transient focal cerebral ischaemia in the freely moving rat was developed in the laboratory. th emodel consist of the injection of endothelin-1, a potent vasoconstrictor, at the level of the arteria cerebri media MCA, causing an infarct in the striatum. Before, during and after the ischaemic damage is assessed by histological examiniation. The effect of drugs on transmitter release and on the infarct volume is poddible predictive for the neuroprotective effect. Striatal blood flow is measured with laser Doppler flowmetry. In this project we aim to test a number of pharmacological strategies for neuroprotection using the endothelin-1 model for focal transient ischaemia. We will focuss on the study of non-NMDA glutamate receptor antagonists, i.e. AMPA and kainate antgonists, group I m GluR antagonists and group II and IIImGluR agonists. Wa era also interseted in the study of adenosine antagonists and we also plan to test combination therapies. We also want to investigate the neuroprotective effect of hypothermia in a model for global ischaemia in the rat. In this model cardiac arrest is induced by anoxia and the rat is subsequently by an inhibition of the release of the neurotransmitters glutamate, GABA and dopamine, which will be measured in the striatum by microdialysis. The neuroprotective efect of the drugs mentioned before will be studied in combination with hypothermia.
AcronymOZR620
StatusFinished
Effective start/end date1/01/0131/12/04

Keywords

  • brain ischaemia
  • animal models
  • pharmacology
  • neurochimistry
  • microdialysis

Flemish discipline codes

  • Biological sciences