Sudy of the Post-polio Syndrome using PCR and cell-free synthesis

  • Rombaut, Bartholomeus, (Administrative Promotor)
  • Vrijsen, Raphael, (Co-Promotor)

Project Details

Description

The disorder of post-polio syndrome (PPS) is undoubtedly as old as paralytic polio itself. There is now sufficient evidence that the persistence of mutated poliovirus in the central nervous sytem might be on the origin of the disorder. The goals of this project are twofold: (1) the further characterisation of the genome of mutants using PCR technology; (2) so far all attempts to isolate virus from cerebrospinal fluid by cell culture remained negative. By using our cell-free system, which can be programmed with only viral RNA, we might be successful in synthesizing the mutant virus. Molecular and biological characterisation is then feasible. As both technologies, PCR for poliovirus and other enteroviruses, and the cellfree system are available and routinely used in our lab, we have the chance of a lifetime to unravel the molecular-biological aspects of the viral origin of the PPS disorder.
AcronymOZR232
StatusFinished
Effective start/end date1/01/9831/12/01

Flemish discipline codes

  • Basic sciences
  • Biological sciences
  • Pharmaceutical sciences

Keywords

  • enterovirus
  • post-polio syndrome
  • mutants
  • PCR