The Interaction Proteome of Oxidative Stress Signalling in Plants

Project Details


Stress in various forms can hinder the expected production level in different agricultural sectors. In contrast to the global climate change the stress are getting new dimensions in their severity and ways such as extreme drought, temperature, salt intrusion, emergence of new pathogens etc. So "stress tolerance" becomes a major objective in biotechnology research. One of the warning messages used by living cell under stress is the production of 'Reactive Oxygen Species' (ROS). ROS functions as a messenger through chemoselective oxidation of cysteine residues in signalling proteins. So, the defense response of cells will be activated by cysteine sulfenylation. If the thiol oxidation state of living cell under stress can be profiled then the signalling proteins could be identified.
The aim of this research project is to identify the proteome of sulfenylated cysteine residues, also called sulfenome. We will use a plant model system that allows modulating in vivo levels of hydrogen peroxide under different stress condition. Previous studies of Oxidative Stress and Cell Death Group (Vandenabeele et al 2004, Vanderauwera et al 2005) made use of catalase 2 deficient plant in Arabidopsis thaliana to study oxidative stress responses. As such, we will profile the thiol oxidation state of A thaliana, (wild type and cat2-mutant) under stress. Through such an approach, we are aiming to identify key signaling proteins that are able to sense changes in the cellular redox state during oxidative stress. Further, we will try to get a clear phenotype of these knockout proteins under high light oxidative stress and their potential as stress tolerance gene will be assessed. Then the proteins will be structurally and functionally analyzed in order to understand the complex networks of interactions induced under cellular redox stress.
Effective start/end date22/11/10 → …


  • Applied Biology