The use of nano LC-MS/MS for the quantification of brain neuropeptides possibly involved in epileptic seizure generation

Project Details


Neuropeptides seem to play an important role when the CNS is challenged. In order to obtain better insights into the central peptidergic effects, it is essential to monitor their concentration in the brain. Quantification of neuropeptides in dialysates is challenging due to their low extracellular concentrations (low pM range), their low microdialysis efficiencies, the need for acceptable temporal resolution, the small sample volumes, the complexity of the matrix and the tendency of peptides to stick to glass and polymeric materials. The quantification of neuropeptides in dialysates therefore necessitates the use of very sensitive nano-LC-MS/MS methods. A number of LC-MS/MS and microdialysis parameters need to be optimized to achieve maximal sensitivity. The optimized and validated methods can be used to investigate the in vivo neuropeptide release during pathological conditions, in this way initiating new and immense challenges for the development of new drugs.
Effective start/end date1/10/1031/12/10

Flemish discipline codes

  • (Bio)chemical engineering
  • Basic sciences
  • Health sciences
  • Pharmaceutical sciences


  • Aminoacids
  • Parkinsons Disease
  • Neuro-Transmitters
  • Neuropharmacology
  • clinical pharmacy
  • Stroke
  • Receptors
  • Rat Models
  • Neurochemistry
  • Pharmacokinetics
  • Microdialysis
  • neuroscience
  • Drug Research
  • Bioanalysis
  • Epilepsy
  • seamless care
  • Monoamines
  • Neurosciences
  • Liquid Chromatography
  • pharmaceutical care
  • Electrochemical Detection