Theiler's murine encephalomyelitis virusinfectie als dierenmodel voor de studie van multiple sclerose

  • Vrijsen, Raphael, (Administrative Promotor)

Project Details

Description

Theiler's murine encephalomyelitis (TME) viruses are divided into 2 groups. One group includes an acute poliomyelitis in mice and the second group induces a chronic, inflammatory infection of the central nerve system (CNS) wich results in demyelination of the neurons. The clinical symptoms and histological lesions induced by the second group of TME viruses in mice are comparable with those of multiple sclerose (MS) in humans. TME virus infection is considered as an excellent animal model for MS. We intend to study the replication (adsorption, uncoating, RNA and protein synthesis and assembly) using the different TME virus strains in a variety of cell structures (neurons, astrocytes and oligodendrocytes). The aim of this study is 1) to compare the replication of the 2 groups of TME viruses and 2) to define the cell types wich gets persistently infected. We also intend to raise monoclonal antibodies (MAb's) against the unknown receptors of TME viruses. The MAb's will be raised using rats and by the same approach wich been succesful in making MAb's against the poliovirus receptor. The MAb's will be used to study the receptor distribution in mouse CNS, and to search for the receptors in mice strains wich are resistant to TME virus infection. Using the CELICS method we intend to define the unknown receptor(s) of TME virus. The method was successfully used to isolate the ECHO virus receptor (1). It's our goal to use TME virus' insensitive cells wich are transformed by cDNA from the TME virus sensitive cells. The transformed cells are selected by MAb against the TME virus receptor. (1): Ward T., P.A. Pipkin, N.A. Clarkson, D.M. Stone, P.D. Minor & J.W. Almond (1994). Decayaccelerating factor CD55 is identified as the receptor for ECHO-virus 7 using CELICS, a rapid immuno-focal cloning method. EMBO Journal, 13, 5070-5074.
AcronymIWT25
StatusFinished
Effective start/end date1/01/9731/12/98

Keywords

  • biomedical sciences
  • virology
  • virus-infections

Flemish discipline codes

  • Basic sciences
  • Biological sciences