Projects per year
Abstract
The cryptic prophage CP-933P in Escherichia coli O157:H7 contains a parDE-like toxin-antitoxin module, the operator region of which is recognized by two flanking transcription regulators: PaaR2 (ParE associated Regulator), which forms part of the paaR2-paaA2-parE2 toxin-antitoxin operon and YdaS (COG4197), which is encoded in the opposite direction but shares the operator. Here we report the 1H, 15N and 13C backbone and side chain chemical shift assignments of YdaS from Escherichia coli O157:H7 in its free state. YdaS is a distinct relative to HigA antitoxins but behaves as a monomer in solution. The BMRB Accession Number is 27917.
Original language | English |
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Pages (from-to) | 25-30 |
Number of pages | 6 |
Journal | Biomolecular NMR Assignments |
Volume | 14 |
Issue number | 1 |
DOIs | |
Publication status | Published - Apr 2020 |
Bibliographical note
Funding Information:This work received financial support from FWO-Vlaanderen (project nr. (G.0226.17N) and the Onderzoeksfonds of the Vrije Universiteit Brussel (OZR-VUB, Grant SPR13).
Funding Information:
This work received financial support from FWO-Vlaanderen (project nr. (G.0226.17N) and the Onderzoeksfonds of the Vrije Universiteit Brussel (OZR-VUB, Grant SPR13).
Publisher Copyright:
© 2019, Springer Nature B.V.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
Keywords
- NMR spectroscopy
- protein structure
- Structural biology
- Toxin-antitoxin module
Fingerprint
Dive into the research topics of '1H, 13C, and 15N backbone and side chain chemical shift assignment of YdaS, a monomeric member of the HigA family'. Together they form a unique fingerprint.Projects
- 2 Finished
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FWOAL831: Regulation of persistence by the paaAR-paaA2-parE2 and cog4197-duf1019 operons from E. coli O157
1/01/17 → 31/12/20
Project: Fundamental
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SRP13: Strategic Research Programme: Structure and dynamics of macromolecular complexes in health and disease
Steyaert, J., Remaut, H. K., Loris, R., Efremov, R., Steyaert, J., Loris, R. & Remaut, H. K.
1/11/12 → 31/10/24
Project: Fundamental
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Bistable expression of a toxin-antitoxin system located in a cryptic prophage of Escherichia coliO157:H7
Jurenas, D., Fraikin, N., Goormaghtigh, F., De Bruyn, P., Vandervelde, A., Jove, T., Charlier, D., Loris, R. & Van Melderen, L., 21 Dec 2021, In: mBio. 12, 6, 15 p., e0294721.Research output: Contribution to journal › Article › peer-review
Open AccessFile12 Citations (Scopus)160 Downloads (Pure) -
Nanobody-aided crystallization of the transcription regulator PaaR2 from Escherichia coli O157:H7
De Bruyn, P., Prolic Kalinsek, M., Vandervelde, A., Malfait, M., Sterckx, Y., Sobott, F., Hadzi, S., Pardon, E., Steyaert, J. & Loris, R., 1 Oct 2021, In: Acta Crystallographica Section F - Structural Biology Communications. 77, 10, p. 374-384 11 p., 11.Research output: Contribution to journal › Article › peer-review
Open AccessFile1 Citation (Scopus)165 Downloads (Pure) -
Toxin-antitoxin modules: functional characterization of transcription regulation and potential use against pathogenic bacteria
De Bruyn, P., 2021, Brussels.Research output: Thesis › PhD Thesis