Projects per year
Abstract
Understanding the kinetics, thermodynamics, and molecular mechanisms of liquid–liquid phase separation (LLPS) is of paramount importance in cell biology, requiring reproducible methods for studying often severely aggregation-prone proteins. Frequently applied approaches for inducing LLPS, such as dilution of the protein from an urea-containing solution or cleavage of its fused solubility tag, often lead to very different kinetic behaviors. Here we demonstrate that at carefully selected pH values proteins such as the low-complexity domain of hnRNPA2, TDP-43, and NUP98, or the stress protein ERD14, can be kept in solution and their LLPS can then be induced by a jump to native pH. This approach represents a generic method for studying the full kinetic trajectory of LLPS under near native conditions that can be easily controlled, providing a platform for the characterization of physiologically relevant phase-separation behavior of diverse proteins.
Original language | English |
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Article number | 77 |
Number of pages | 8 |
Journal | Communications Biology |
Volume | 4 |
Issue number | 1 |
DOIs | |
Publication status | Published - 19 Jan 2021 |
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Dive into the research topics of 'A generic approach to study the kinetics of liquid–liquid phase separation under near-native conditions'. Together they form a unique fingerprint.Projects
- 1 Finished
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SRP51: SRP-Onderzoekszwaartepunt: Optical and microfluidic tools to unravel the dynamics of bio-condensates
De Malsche, W., Ottevaere, H., Maes, D. & Tompa, P.
1/03/19 → 29/02/24
Project: Fundamental
Activities
- 1 Talk or presentation at a conference
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PhasAGE International Conference
Luis Fernando Duran Armenta (Speaker)
12 Oct 2021 → 14 Oct 2021Activity: Talk or presentation › Talk or presentation at a conference