A metagenome-wide association study of gut microbiota in type 2 diabetes

Junjie Qin; Li Yingrui; Zhiming Cai; Shenghui Li; Jianfeng Zhu; Fan Zhang; Suisha Liang; Wenwei Zhang; Yuanlin Guan; Dongqian Shen; Yangqing Peng; Dongya Zhang; Zhuye Jie; Wenxian Wu; Youwen Qin; Wenbin Xue; Junhua Li; Lingchuan Han; Donghui Lu; Peixian Xu; Yali Dai; Xiaojuan Sun; Zesong Li; Aifa Tang; Shilong Zhong; Xiaoping Li; Weineng Chen; Ran Xu; Mingbang Wang; Qiang Feng; Meihua Gong; Jing Yu; Yanyan Zhang; Ming Zhang; Torben Hansen; Gaston Sanchez; Jeroen Raes; Gwen Falony; Okuda Shujiro; Mathieu Almeida; Emmanuelle Lechatelier; Pierre Renault; Nicolas Pons; Jean-Michel Batto; Z Zhang; H. Chen; Ruifu Yang; Weimou Zheng; Songgang Li; Huanming Yang; Jian Wang; Ehrlich Dusko; Rasmus Nielsen; Oluf Pedersen; Karsten Kristiansen; Jun Wang

A metagenome-wide association study of gut microbiota in type 2 diabetes

Junjie Qin, Li Yingrui, Zhiming Cai, Shenghui Li, Jianfeng Zhu, Fan Zhang, Suisha Liang, Wenwei Zhang, Yuanlin Guan, Dongqian Shen, Yangqing Peng, Dongya Zhang, Zhuye Jie, Wenxian Wu, Youwen Qin, Wenbin Xue, Junhua Li, Lingchuan Han, Donghui Lu, Peixian XuYali Dai, Xiaojuan Sun, Zesong Li, Aifa Tang, Shilong Zhong, Xiaoping Li, Weineng Chen, Ran Xu, Mingbang Wang, Qiang Feng, Meihua Gong, Jing Yu, Yanyan Zhang, Ming Zhang, Torben Hansen, Gaston Sanchez, Jeroen Raes, Gwen Falony, Okuda Shujiro, Mathieu Almeida, Emmanuelle Lechatelier, Pierre Renault, Nicolas Pons, Jean-Michel Batto, Z Zhang, H. Chen, Ruifu Yang, Weimou Zheng, Songgang Li, Huanming Yang, Jian Wang, Ehrlich Dusko, Rasmus Nielsen, Oluf Pedersen, Karsten Kristiansen, Jun Wang

Department of Bio-engineering Sciences

Research output: Contribution to journal › Article › peer-review

5578 Citations (Scopus)

Abstract

Assessment and characterization of gut microbiota has become a major research area in human disease, including type 2 diabetes, the most prevalent endocrine disease worldwide. To carry out analysis on gut microbial content in patients with type 2 diabetes, we developed a protocol for a metagenome-wide association study (MGWAS) and undertook a two-stage MGWAS based on deep shotgun sequencing of the gut microbial DNA from 345 Chinese individuals. We identified and validated approximately 60,000 type-2-diabetes-associated markers and established the concept of a metagenomic linkage group, enabling taxonomic species-level analyses. MGWAS analysis showed that patients with type 2 diabetes were characterized by a moderate degree of gut microbial dysbiosis, a decrease in the abundance of some universal butyrate-producing bacteria and an increase in various opportunistic pathogens, as well as an enrichment of other microbial functions conferring sulphate reduction and oxidative stress resistance. An analysis of 23 additional individuals demonstrated that these gut microbial markers might be useful for classifying type 2 diabetes.

Original language	English
Pages (from-to)	55-60
Number of pages	6
Journal	Nature
Volume	490
Publication status	Published - 4 Oct 2012

Keywords

gut microbiota, diabetes

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@article{15247928f8ef4fd896a5406af212dbdb,

title = "A metagenome-wide association study of gut microbiota in type 2 diabetes",

abstract = "Assessment and characterization of gut microbiota has become a major research area in human disease, including type 2 diabetes, the most prevalent endocrine disease worldwide. To carry out analysis on gut microbial content in patients with type 2 diabetes, we developed a protocol for a metagenome-wide association study (MGWAS) and undertook a two-stage MGWAS based on deep shotgun sequencing of the gut microbial DNA from 345 Chinese individuals. We identified and validated approximately 60,000 type-2-diabetes-associated markers and established the concept of a metagenomic linkage group, enabling taxonomic species-level analyses. MGWAS analysis showed that patients with type 2 diabetes were characterized by a moderate degree of gut microbial dysbiosis, a decrease in the abundance of some universal butyrate-producing bacteria and an increase in various opportunistic pathogens, as well as an enrichment of other microbial functions conferring sulphate reduction and oxidative stress resistance. An analysis of 23 additional individuals demonstrated that these gut microbial markers might be useful for classifying type 2 diabetes.",

keywords = "gut microbiota, diabetes",

author = "Junjie Qin and Li Yingrui and Zhiming Cai and Shenghui Li and Jianfeng Zhu and Fan Zhang and Suisha Liang and Wenwei Zhang and Yuanlin Guan and Dongqian Shen and Yangqing Peng and Dongya Zhang and Zhuye Jie and Wenxian Wu and Youwen Qin and Wenbin Xue and Junhua Li and Lingchuan Han and Donghui Lu and Peixian Xu and Yali Dai and Xiaojuan Sun and Zesong Li and Aifa Tang and Shilong Zhong and Xiaoping Li and Weineng Chen and Ran Xu and Mingbang Wang and Qiang Feng and Meihua Gong and Jing Yu and Yanyan Zhang and Ming Zhang and Torben Hansen and Gaston Sanchez and Jeroen Raes and Gwen Falony and Okuda Shujiro and Mathieu Almeida and Emmanuelle Lechatelier and Pierre Renault and Nicolas Pons and Jean-Michel Batto and Z Zhang and H. Chen and Ruifu Yang and Weimou Zheng and Songgang Li and Huanming Yang and Jian Wang and Ehrlich Dusko and Rasmus Nielsen and Oluf Pedersen and Karsten Kristiansen and Jun Wang",

year = "2012",

month = oct,

day = "4",

language = "English",

volume = "490",

pages = "55--60",

journal = "Nature",

issn = "0028-0836",

publisher = "Nature Publishing Group",

}

Qin, J, Yingrui, L, Cai, Z, Li, S, Zhu, J, Zhang, F, Liang, S, Zhang, W, Guan, Y, Shen, D, Peng, Y, Zhang, D, Jie, Z, Wu, W, Qin, Y, Xue, W, Li, J, Han, L, Lu, D, Xu, P, Dai, Y, Sun, X, Li, Z, Tang, A, Zhong, S, Li, X, Chen, W, Xu, R, Wang, M, Feng, Q, Gong, M, Yu, J, Zhang, Y, Zhang, M, Hansen, T, Sanchez, G, Raes, J, Falony, G, Shujiro, O, Almeida, M, Lechatelier, E, Renault, P, Pons, N, Batto, J-M, Zhang, Z, Chen, H, Yang, R, Zheng, W, Li, S, Yang, H, Wang, J, Dusko, E, Nielsen, R, Pedersen, O, Kristiansen, K & Wang, J 2012, 'A metagenome-wide association study of gut microbiota in type 2 diabetes', Nature, vol. 490, pp. 55-60.

TY - JOUR

T1 - A metagenome-wide association study of gut microbiota in type 2 diabetes

AU - Qin, Junjie

AU - Yingrui, Li

AU - Cai, Zhiming

AU - Li, Shenghui

AU - Zhu, Jianfeng

AU - Zhang, Fan

AU - Liang, Suisha

AU - Zhang, Wenwei

AU - Guan, Yuanlin

AU - Shen, Dongqian

AU - Peng, Yangqing

AU - Zhang, Dongya

AU - Jie, Zhuye

AU - Wu, Wenxian

AU - Qin, Youwen

AU - Xue, Wenbin

AU - Li, Junhua

AU - Han, Lingchuan

AU - Lu, Donghui

AU - Xu, Peixian

AU - Dai, Yali

AU - Sun, Xiaojuan

AU - Li, Zesong

AU - Tang, Aifa

AU - Zhong, Shilong

AU - Li, Xiaoping

AU - Chen, Weineng

AU - Xu, Ran

AU - Wang, Mingbang

AU - Feng, Qiang

AU - Gong, Meihua

AU - Yu, Jing

AU - Zhang, Yanyan

AU - Zhang, Ming

AU - Hansen, Torben

AU - Sanchez, Gaston

AU - Raes, Jeroen

AU - Falony, Gwen

AU - Shujiro, Okuda

AU - Almeida, Mathieu

AU - Lechatelier, Emmanuelle

AU - Renault, Pierre

AU - Pons, Nicolas

AU - Batto, Jean-Michel

AU - Zhang, Z

AU - Chen, H.

AU - Yang, Ruifu

AU - Zheng, Weimou

AU - Li, Songgang

AU - Yang, Huanming

AU - Wang, Jian

AU - Dusko, Ehrlich

AU - Nielsen, Rasmus

AU - Pedersen, Oluf

AU - Kristiansen, Karsten

AU - Wang, Jun

PY - 2012/10/4

Y1 - 2012/10/4

N2 - Assessment and characterization of gut microbiota has become a major research area in human disease, including type 2 diabetes, the most prevalent endocrine disease worldwide. To carry out analysis on gut microbial content in patients with type 2 diabetes, we developed a protocol for a metagenome-wide association study (MGWAS) and undertook a two-stage MGWAS based on deep shotgun sequencing of the gut microbial DNA from 345 Chinese individuals. We identified and validated approximately 60,000 type-2-diabetes-associated markers and established the concept of a metagenomic linkage group, enabling taxonomic species-level analyses. MGWAS analysis showed that patients with type 2 diabetes were characterized by a moderate degree of gut microbial dysbiosis, a decrease in the abundance of some universal butyrate-producing bacteria and an increase in various opportunistic pathogens, as well as an enrichment of other microbial functions conferring sulphate reduction and oxidative stress resistance. An analysis of 23 additional individuals demonstrated that these gut microbial markers might be useful for classifying type 2 diabetes.

AB - Assessment and characterization of gut microbiota has become a major research area in human disease, including type 2 diabetes, the most prevalent endocrine disease worldwide. To carry out analysis on gut microbial content in patients with type 2 diabetes, we developed a protocol for a metagenome-wide association study (MGWAS) and undertook a two-stage MGWAS based on deep shotgun sequencing of the gut microbial DNA from 345 Chinese individuals. We identified and validated approximately 60,000 type-2-diabetes-associated markers and established the concept of a metagenomic linkage group, enabling taxonomic species-level analyses. MGWAS analysis showed that patients with type 2 diabetes were characterized by a moderate degree of gut microbial dysbiosis, a decrease in the abundance of some universal butyrate-producing bacteria and an increase in various opportunistic pathogens, as well as an enrichment of other microbial functions conferring sulphate reduction and oxidative stress resistance. An analysis of 23 additional individuals demonstrated that these gut microbial markers might be useful for classifying type 2 diabetes.

KW - gut microbiota, diabetes

M3 - Article

SN - 0028-0836

VL - 490

SP - 55

EP - 60

JO - Nature

JF - Nature

ER -

A metagenome-wide association study of gut microbiota in type 2 diabetes

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