A universal fixation method based on quaternary ammonium salts (RNAlater) for omics-technologies: Saccharomyces cerevisiae as a case study.

Katty Goossens, Rudy Van Eijsden, Catherine Stassen, Luk Daenen, Sebastiaan Van Mulders, Prashant Bapat, Verena Siewers, J. Nielsen, Freddy R Delvaux, P. Van Hummelen, Bart Devreese, Ronnie Willaert

Research output: Chapter in Book/Report/Conference proceedingMeeting abstract (Book)

12 Citations (Scopus)

Abstract

RNAlater is an excellent storage agent for yeast cells and, most likely, also for other cell types and tissues, in order to preserve the samples for later subjection to the major omics-techniques and microscopic analyses. Moreover, yeast cells remained viable after preservation with RNAlater, making it a good alternative when other storage methods, often using liquid N2, are not available. For these reasons, RNAlater is in particular of interest for field experiments or experiments in special environments, such as during microgravity research.
Original languageEnglish
Title of host publicationUK Astrobiology Conference, Edingburgh, UK, April 17-19.
Publication statusPublished - 17 Apr 2013
EventUnknown -
Duration: 17 Apr 2013 → …

Conference

ConferenceUnknown
Period17/04/13 → …

Keywords

  • Saccharomyces cerevisiae
  • omics
  • RNAlater
  • microgravity
  • proteomics
  • metabolic flux analysis
  • microarray analysis
  • fluorescence microscopy

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