Projects per year
Objective: To address the early dynamics of LB secretion, we examined cytoskeletal remodeling of keratinocytes in 3 mouse models following acute barrier abrogation: hairless mice, PAR-2 knockout (-/-) and cav-1 -/-.
Methods and results: Under basal conditions, globular (G)-actin accumulates in SG cells cytosol, while filamentous (F)-actin is restricted to pen-membrane domains. Barrier abrogation induces the apical movement of F-actin and the retreat of the SG-G-actin front, paralleled by upstream cytoskeletal kinases activation. This phenomenon was both enhanced by PAR-2 agonist, and inhibited by cytochalasin-D and in PAR-2 knockout mice. We found that plasma membrane conformational changes causing LB secretion are controlled by PAR-2-dependent cytoskeletal rearrangements. We next addressed the interaction dynamics between cytoskeleton and plasma membrane following PAR-2-induced actin stress fiber formation in both cav-1 -/- and wildtype cells. Actin stress fiber formation is increased in cav-1 -/- cells prior to and following PAR-2 agonist peptide-treatment, while absence of cav-1 inhibits E-cadherin-mediated cell-to-cell adhesion.
Conclusion: PAR-2 drives cytoskeletal/plasma membrane dynamics that regulate early LB secretion following barrier abrogation, stress fiber formation and keratinocyte adhesion. (C) 2010 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.
|Number of pages||10|
|Journal||Journal of Dermatological Science|
|Publication status||Published - 2011|
- epidermal permeability
- PROTEINASE-ACTIVATED RECEPTOR-2
- HUMAN KERATINOCYTES
FingerprintDive into the research topics of 'Actin dynamics regulate immediate PAR-2-dependent responses to acute epidermal permeability barrier abrogation'. Together they form a unique fingerprint.
- 1 Finished
FWOAL399: Unraveling the pathways of permeability Barrier homeostasis: the cross-talk between corneum stress events and signalling transduction within the viable epidermis.
1/01/07 → 31/12/10