Adjuvant-Enhanced mRNA Vaccines

Lukasz Bialkowski; Kevin Van der Jeught; Dries Renmans; Alexia van Weijnen; Carlo Heirman; Marleen Keyaerts; Karine Breckpot; Kris Thielemans

doi:10.1007/978-1-4939-6481-9_11

Adjuvant-Enhanced mRNA Vaccines

Lukasz Bialkowski, Kevin Van der Jeught, Dries Renmans, Alexia van Weijnen, Carlo Heirman, Marleen Keyaerts, Karine Breckpot, Kris Thielemans

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Recent advances in molecular biology have led to dramatic enhancement of the stability of in vitro transcribed (IVT) messenger RNA (mRNA) and improvement in its translational efficacy. Nowadays, mRNA-based vaccines represent a promising approach in the field of anticancer immunotherapy, gaining attention over the earlier-established bacteria-, virus-, or cell-based vaccination approaches. Here, we present the experimental procedures employed in our laboratory to induce anticancer immune responses in different murine tumor models using IVT mRNA encoding for immune activation signals and antigens of interest.

Original language	English
Pages (from-to)	179-191
Number of pages	13
Journal	Methods in Molecular Biology
Volume	1499
DOIs	https://doi.org/10.1007/978-1-4939-6481-9_11
Publication status	Published - 2017

Keywords

Bioluminescence imaging
Cancer immunotherapy
Dendritic cells
Electroporation
Intralymphatic
Intranodal
Intratumoral
Therapeutic vaccine
mRNA

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10.1007/978-1-4939-6481-9_11

Cite this

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title = "Adjuvant-Enhanced mRNA Vaccines",

abstract = "Recent advances in molecular biology have led to dramatic enhancement of the stability of in vitro transcribed (IVT) messenger RNA (mRNA) and improvement in its translational efficacy. Nowadays, mRNA-based vaccines represent a promising approach in the field of anticancer immunotherapy, gaining attention over the earlier-established bacteria-, virus-, or cell-based vaccination approaches. Here, we present the experimental procedures employed in our laboratory to induce anticancer immune responses in different murine tumor models using IVT mRNA encoding for immune activation signals and antigens of interest.",

keywords = "Bioluminescence imaging, Cancer immunotherapy, Dendritic cells, Electroporation, Intralymphatic, Intranodal, Intratumoral, Therapeutic vaccine, mRNA",

author = "Lukasz Bialkowski and {Van der Jeught}, Kevin and Dries Renmans and {van Weijnen}, Alexia and Carlo Heirman and Marleen Keyaerts and Karine Breckpot and Kris Thielemans",

year = "2017",

doi = "10.1007/978-1-4939-6481-9_11",

language = "English",

volume = "1499",

pages = "179--191",

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T1 - Adjuvant-Enhanced mRNA Vaccines

AU - Bialkowski, Lukasz

AU - Van der Jeught, Kevin

AU - Renmans, Dries

AU - van Weijnen, Alexia

AU - Heirman, Carlo

AU - Keyaerts, Marleen

AU - Breckpot, Karine

AU - Thielemans, Kris

PY - 2017

Y1 - 2017

N2 - Recent advances in molecular biology have led to dramatic enhancement of the stability of in vitro transcribed (IVT) messenger RNA (mRNA) and improvement in its translational efficacy. Nowadays, mRNA-based vaccines represent a promising approach in the field of anticancer immunotherapy, gaining attention over the earlier-established bacteria-, virus-, or cell-based vaccination approaches. Here, we present the experimental procedures employed in our laboratory to induce anticancer immune responses in different murine tumor models using IVT mRNA encoding for immune activation signals and antigens of interest.

AB - Recent advances in molecular biology have led to dramatic enhancement of the stability of in vitro transcribed (IVT) messenger RNA (mRNA) and improvement in its translational efficacy. Nowadays, mRNA-based vaccines represent a promising approach in the field of anticancer immunotherapy, gaining attention over the earlier-established bacteria-, virus-, or cell-based vaccination approaches. Here, we present the experimental procedures employed in our laboratory to induce anticancer immune responses in different murine tumor models using IVT mRNA encoding for immune activation signals and antigens of interest.

KW - Bioluminescence imaging

KW - Cancer immunotherapy

KW - Dendritic cells

KW - Electroporation

KW - Intralymphatic

KW - Intranodal

KW - Intratumoral

KW - Therapeutic vaccine

KW - mRNA

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DO - 10.1007/978-1-4939-6481-9_11

M3 - Article

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VL - 1499

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EP - 191

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

SN - 1064-3745

ER -

Adjuvant-Enhanced mRNA Vaccines

Abstract

Keywords

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SRP48: Strategic Research Programme: Cancer Cell Targeting in Myeloma and Melanoma (MyMe)

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Adjuvant-Enhanced mRNA Vaccines

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Projects

SRP48: Strategic Research Programme: Cancer Cell Targeting in Myeloma and Melanoma (MyMe)

Cite this