Projects per year
Abstract
Toxin-antitoxin (TA) modules are small operons associated with stress response of bacteria. F-plasmid CcdBF was the first TA toxin for which its target, gyrase, was identified. Plasmidic and chromosomal CcdB's form distinct families. Conserved residues crucial for gyrase poisoning activity of plasmidic CcdB's are not conserved among these families. Here we show that the chromosomal CcdBVfi from Vibrio fischeri is an active gyrase poison that interacts with its target via an alternative energetic mechanism. Changes in the GyrA14 binding surface of the Vibrio and F-plasmid CcdB family members illustrate neutral drift where alternative interactions can be used to achieve the same functionality. Differences in affinity between V. fischeri and F-plasmid CcdB for gyrase and their corresponding CcdA antitoxin possibly reflect distinct roles for TA modules located on plasmids and chromosomes.
Original language | English |
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Pages (from-to) | 965-978 |
Number of pages | 14 |
Journal | Mol. Microbiol. |
Volume | 84 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2012 |
Keywords
- structural biology
- complementary methods in structural biology
- biophysics
- toxin-antitoxin module
- gyrase poison
- plasmid addiction
- protein-protein interaction
- bacterial stress response
- bacterial toxin
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Dive into the research topics of 'Alternative interactions define Gyrase specificity in the CcdB family'. Together they form a unique fingerprint.Projects
- 4 Finished
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SRP13: Strategic Research Programme: Structure and dynamics of macromolecular complexes in health and disease
Steyaert, J., Remaut, H. K., Loris, R., Efremov, R., Steyaert, J., Loris, R. & Remaut, H. K.
1/11/12 → 31/10/24
Project: Fundamental
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FWOAL381: Structural basis of plasmide addiction and programed cell death in bacteria
Haesaerts, S., De Greve, H. & Loris, R.
1/01/06 → 31/12/09
Project: Fundamental
Activities
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Modern Biophysical Techniques for the Life Sciences
Yann Sterckx (Participant)
20 Oct 2014 → 21 Oct 2014Activity: Participating in or organising an event › Participation in conference
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Modern Biophysical Techniques for the Life Sciences
Valentina Zorzini (Participant)
20 Oct 2014 → 21 Oct 2014Activity: Participating in or organising an event › Participation in conference
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Modern Biophysical Techniques for the Life Sciences
Steven De Gieter (Participant)
20 Oct 2014 → 21 Oct 2014Activity: Participating in or organising an event › Participation in conference