An ultrasensitive nano UHPLC-ESI-MS/MS method for the quantification of three neuromedin-like peptides in microdialysates

Katrien Maes; Guillaume Béchade; Jolien Van Schoors; Yannick Van Wanseele; Joeri Van Liefferinge; Yvette Michotte; Stephanie N Harden; Erin E Chambers; Jan Claereboudt; Ilse Smolders; Ann Van Eeckhaut

doi:10.4155/bio.14.269

An ultrasensitive nano UHPLC-ESI-MS/MS method for the quantification of three neuromedin-like peptides in microdialysates

Katrien Maes, Guillaume Béchade, Jolien Van Schoors, Yannick Van Wanseele, Joeri Van Liefferinge, Yvette Michotte, Stephanie N Harden, Erin E Chambers, Jan Claereboudt, Ilse Smolders, Ann Van Eeckhaut

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

AIM: An ultrasensitive nano UHPLC-ESI-MS/MS method is developed to simultaneously monitor three low-concentration neuromedin-like peptides in microdialysates.

RESULTS: Peptide preconcentration and sample desalting is performed online on a trap column. A shallow gradient slope at 300 nl/min on the analytical column maintained at 35°C, followed by two saw-tooth column wash cycles, results in the highest sensitivity and the lowest carryover. The validated method allows the accurate and precise quantification of 0.5 pM neurotensin and neuromedin N (2.5 amol on column), and of 3.0 pM neuromedin B (15.0 amol on column) in in vivo microdialysates without the use of internal standards.

CONCLUSION: The assay is an important tool for elucidating the role of these neuromedin-like peptides in the pathophysiology of neurological disorders.

Original language	English
Pages (from-to)	605-619
Number of pages	15
Journal	Bioanalysis
Volume	7
Issue number	5
DOIs	https://doi.org/10.4155/bio.14.269
Publication status	Published - 2015

Keywords

Chromatography, Liquid
Microdialysis
Neurotensin
Peptide Fragments
Peptides
Tandem Mass Spectrometry

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10.4155/bio.14.269

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Maes, Katrien ; Béchade, Guillaume ; Van Schoors, Jolien ; Van Wanseele, Yannick ; Van Liefferinge, Joeri ; Michotte, Yvette ; Harden, Stephanie N ; Chambers, Erin E ; Claereboudt, Jan ; Smolders, Ilse ; Van Eeckhaut, Ann. / An ultrasensitive nano UHPLC-ESI-MS/MS method for the quantification of three neuromedin-like peptides in microdialysates. In: Bioanalysis. 2015 ; Vol. 7, No. 5. pp. 605-619.

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title = "An ultrasensitive nano UHPLC-ESI-MS/MS method for the quantification of three neuromedin-like peptides in microdialysates",

abstract = "AIM: An ultrasensitive nano UHPLC-ESI-MS/MS method is developed to simultaneously monitor three low-concentration neuromedin-like peptides in microdialysates.RESULTS: Peptide preconcentration and sample desalting is performed online on a trap column. A shallow gradient slope at 300 nl/min on the analytical column maintained at 35°C, followed by two saw-tooth column wash cycles, results in the highest sensitivity and the lowest carryover. The validated method allows the accurate and precise quantification of 0.5 pM neurotensin and neuromedin N (2.5 amol on column), and of 3.0 pM neuromedin B (15.0 amol on column) in in vivo microdialysates without the use of internal standards.CONCLUSION: The assay is an important tool for elucidating the role of these neuromedin-like peptides in the pathophysiology of neurological disorders.",

keywords = "Chromatography, Liquid, Microdialysis, Neurotensin, Peptide Fragments, Peptides, Tandem Mass Spectrometry",

author = "Katrien Maes and Guillaume B{\'e}chade and {Van Schoors}, Jolien and {Van Wanseele}, Yannick and {Van Liefferinge}, Joeri and Yvette Michotte and Harden, {Stephanie N} and Chambers, {Erin E} and Jan Claereboudt and Ilse Smolders and {Van Eeckhaut}, Ann",

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doi = "10.4155/bio.14.269",

language = "English",

volume = "7",

pages = "605--619",

journal = "Bioanalysis",

issn = "1757-6180",

publisher = "Future Science",

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An ultrasensitive nano UHPLC-ESI-MS/MS method for the quantification of three neuromedin-like peptides in microdialysates. / Maes, Katrien; Béchade, Guillaume; Van Schoors, Jolien; Van Wanseele, Yannick; Van Liefferinge, Joeri; Michotte, Yvette; Harden, Stephanie N; Chambers, Erin E; Claereboudt, Jan; Smolders, Ilse ; Van Eeckhaut, Ann.

In: Bioanalysis, Vol. 7, No. 5, 2015, p. 605-619.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - An ultrasensitive nano UHPLC-ESI-MS/MS method for the quantification of three neuromedin-like peptides in microdialysates

AU - Maes, Katrien

AU - Béchade, Guillaume

AU - Van Schoors, Jolien

AU - Van Wanseele, Yannick

AU - Van Liefferinge, Joeri

AU - Michotte, Yvette

AU - Harden, Stephanie N

AU - Chambers, Erin E

AU - Claereboudt, Jan

AU - Smolders, Ilse

AU - Van Eeckhaut, Ann

PY - 2015

Y1 - 2015

N2 - AIM: An ultrasensitive nano UHPLC-ESI-MS/MS method is developed to simultaneously monitor three low-concentration neuromedin-like peptides in microdialysates.RESULTS: Peptide preconcentration and sample desalting is performed online on a trap column. A shallow gradient slope at 300 nl/min on the analytical column maintained at 35°C, followed by two saw-tooth column wash cycles, results in the highest sensitivity and the lowest carryover. The validated method allows the accurate and precise quantification of 0.5 pM neurotensin and neuromedin N (2.5 amol on column), and of 3.0 pM neuromedin B (15.0 amol on column) in in vivo microdialysates without the use of internal standards.CONCLUSION: The assay is an important tool for elucidating the role of these neuromedin-like peptides in the pathophysiology of neurological disorders.

AB - AIM: An ultrasensitive nano UHPLC-ESI-MS/MS method is developed to simultaneously monitor three low-concentration neuromedin-like peptides in microdialysates.RESULTS: Peptide preconcentration and sample desalting is performed online on a trap column. A shallow gradient slope at 300 nl/min on the analytical column maintained at 35°C, followed by two saw-tooth column wash cycles, results in the highest sensitivity and the lowest carryover. The validated method allows the accurate and precise quantification of 0.5 pM neurotensin and neuromedin N (2.5 amol on column), and of 3.0 pM neuromedin B (15.0 amol on column) in in vivo microdialysates without the use of internal standards.CONCLUSION: The assay is an important tool for elucidating the role of these neuromedin-like peptides in the pathophysiology of neurological disorders.

KW - Chromatography, Liquid

KW - Microdialysis

KW - Neurotensin

KW - Peptide Fragments

KW - Peptides

KW - Tandem Mass Spectrometry

U2 - 10.4155/bio.14.269

DO - 10.4155/bio.14.269

M3 - Article

C2 - 25826142

VL - 7

SP - 605

EP - 619

JO - Bioanalysis

JF - Bioanalysis

SN - 1757-6180

IS - 5

ER -

An ultrasensitive nano UHPLC-ESI-MS/MS method for the quantification of three neuromedin-like peptides in microdialysates

Abstract

Keywords

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