An ultrasensitive nano UHPLC-ESI-MS/MS method for the quantification of three neuromedin-like peptides in microdialysates

Katrien Maes, Guillaume Béchade, Jolien Van Schoors, Yannick Van Wanseele, Joeri Van Liefferinge, Yvette Michotte, Stephanie N Harden, Erin E Chambers, Jan Claereboudt, Ilse Smolders, Ann Van Eeckhaut

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

AIM: An ultrasensitive nano UHPLC-ESI-MS/MS method is developed to simultaneously monitor three low-concentration neuromedin-like peptides in microdialysates.

RESULTS: Peptide preconcentration and sample desalting is performed online on a trap column. A shallow gradient slope at 300 nl/min on the analytical column maintained at 35°C, followed by two saw-tooth column wash cycles, results in the highest sensitivity and the lowest carryover. The validated method allows the accurate and precise quantification of 0.5 pM neurotensin and neuromedin N (2.5 amol on column), and of 3.0 pM neuromedin B (15.0 amol on column) in in vivo microdialysates without the use of internal standards.

CONCLUSION: The assay is an important tool for elucidating the role of these neuromedin-like peptides in the pathophysiology of neurological disorders.

Original languageEnglish
Pages (from-to)605-619
Number of pages15
JournalBioanalysis
Volume7
Issue number5
DOIs
Publication statusPublished - 2015

Keywords

  • Chromatography, Liquid
  • Microdialysis
  • Neurotensin
  • Peptide Fragments
  • Peptides
  • Tandem Mass Spectrometry

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