Analysis of a rare melanoma patient with a spontaneous CTL response to a MAGE-A3 peptide presented by HLA-A1

Takeshi Hanagiri, Nicolas van Baren, Bart Neyns, Thierry Boon, Pierre G Coulie

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We describe an HLA-A1 melanoma patient who has mounted a spontaneous cytolytic T cell (CTL) response against an antigenic peptide encoded by gene MAGE-A3 and presented by HLA-A1. The frequency of anti-MAGE-3.A1 CTLp was 5 x 10(-7) of the blood CD8 cells, with a dominant clonotype which was present in six out of seven independent anti-MAGE-3.A1 CTL clones. After vaccination with a recombinant poxvirus coding for the MAGE-3.A1 antigen, the blood frequency of anti-MAGE-3.A1 CTLp increased tenfold. Twenty-two independent CTL clones were derived. Surprisingly, only one of them corresponded to the dominant clonotype present before vaccination. Two new clonotypes were repeated 12 and 7 times, respectively. Our interpretation of these results is that the spontaneous anti-MAGE-3.A1 CTL response pre-existing to vaccination was polyclonal, and that the vaccine restimulated only some of these clones. To estimate the incidence of spontaneous anti-MAGE-3.A1 CTL responses in melanoma patients with a tumor expressing gene MAGE-A3, we measured the blood frequency of anti-MAGE-3.A1 T cells in 45 patients, and found only two clear responses.

Original languageEnglish
Pages (from-to)178-184
Number of pages7
JournalCancer Immunol Immunother
Volume55
Issue number2
DOIs
Publication statusPublished - Feb 2006

Keywords

  • Aged
  • Antigens, Neoplasm/administration & dosage
  • Cancer Vaccines/administration & dosage
  • Fatal Outcome
  • Female
  • HLA-A1 Antigen/immunology
  • Humans
  • Melanoma/drug therapy
  • Neoplasm Proteins/administration & dosage
  • T-Lymphocytes, Cytotoxic/immunology
  • Treatment Failure

Fingerprint Dive into the research topics of 'Analysis of a rare melanoma patient with a spontaneous CTL response to a MAGE-A3 peptide presented by HLA-A1'. Together they form a unique fingerprint.

Cite this