Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction

Michaël Maes; Joost Willebrords; Sara Crespo Yanguas; Bruno Cogliati; Mathieu Vinken

Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction

Michaël Maes, Joost Willebrords, Sara Crespo Yanguas, Bruno Cogliati, Mathieu Vinken

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

7 Citations (Scopus)

Abstract

Although connexin production is mainly regulated at the protein level, altered connexin gene expression has been identified as the underlying mechanism of several pathologies. When studying the latter, appropriate methods to quantify connexin mRNA levels are required. The present chapter describes a well-established reverse transcription quantitative real-time polymerase chain reaction procedure optimized for analysis of hepatic connexins. The method includes RNA extraction and subsequent quantification, generation of complementary DNA, quantitative real-time polymerase chain reaction and data analysis.

Original language	English
Title of host publication	Gap Junction Protocols
Editors	Mathieu Vinken, Scott Johnstone
Place of Publication	New York
Publisher	Springer Protocols
Pages	1-19
ISBN (Print)	978-1-4939-3664-9
Publication status	Published - 22 May 2016

Keywords

Connexin
RNA extraction
reverse transcription

1 Active
2 Finished

OZR2651: International Joint Research Group - Liver Connexin and Pannexin Research Group (LCPR)
Vinken, M., Rogiers, V., Cogliati, B. & Dagli, M. L.
1/08/14 → 1/07/26
Project: Fundamental
EU457: CONNECT: Connexin and pannexin channels as drug targets and biomarkers in acute and chronic liver disease
Vinken, M.
1/03/14 → 28/02/19
Project: Fundamental
IWT680: Characterisatin of connexin43 and pannexin1 as well as corresponding hemichannels as biomarkers and pharmacological targets in acute liver failure: a combined vitro-in vivo study
Vinken, M.
1/01/14 → 31/12/17
Project: Fundamental

The role of connexins, pannexins and their channels in liver fibrosis
Sara Crespo Yanguas (Speaker)
28 Nov 2018
Activity: Talk or presentation › Talk or presentation at a workshop/seminar

Cite this

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title = "Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction",

abstract = "Although connexin production is mainly regulated at the protein level, altered connexin gene expression has been identified as the underlying mechanism of several pathologies. When studying the latter, appropriate methods to quantify connexin mRNA levels are required. The present chapter describes a well-established reverse transcription quantitative real-time polymerase chain reaction procedure optimized for analysis of hepatic connexins. The method includes RNA extraction and subsequent quantification, generation of complementary DNA, quantitative real-time polymerase chain reaction and data analysis.",

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author = "Micha{\"e}l Maes and Joost Willebrords and {Crespo Yanguas}, Sara and Bruno Cogliati and Mathieu Vinken",

year = "2016",

month = may,

day = "22",

language = "English",

isbn = "978-1-4939-3664-9",

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Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction. / Maes, Michaël; Willebrords, Joost; Crespo Yanguas, Sara; Cogliati, Bruno; Vinken, Mathieu.

Gap Junction Protocols. ed. / Mathieu Vinken; Scott Johnstone. New York : Springer Protocols, 2016. p. 1-19.

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

TY - CHAP

T1 - Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction

AU - Maes, Michaël

AU - Willebrords, Joost

AU - Crespo Yanguas, Sara

AU - Cogliati, Bruno

AU - Vinken, Mathieu

PY - 2016/5/22

Y1 - 2016/5/22

N2 - Although connexin production is mainly regulated at the protein level, altered connexin gene expression has been identified as the underlying mechanism of several pathologies. When studying the latter, appropriate methods to quantify connexin mRNA levels are required. The present chapter describes a well-established reverse transcription quantitative real-time polymerase chain reaction procedure optimized for analysis of hepatic connexins. The method includes RNA extraction and subsequent quantification, generation of complementary DNA, quantitative real-time polymerase chain reaction and data analysis.

AB - Although connexin production is mainly regulated at the protein level, altered connexin gene expression has been identified as the underlying mechanism of several pathologies. When studying the latter, appropriate methods to quantify connexin mRNA levels are required. The present chapter describes a well-established reverse transcription quantitative real-time polymerase chain reaction procedure optimized for analysis of hepatic connexins. The method includes RNA extraction and subsequent quantification, generation of complementary DNA, quantitative real-time polymerase chain reaction and data analysis.

KW - Connexin

KW - RNA extraction

KW - reverse transcription

M3 - Chapter

SN - 978-1-4939-3664-9

SP - 1

EP - 19

BT - Gap Junction Protocols

A2 - Vinken, Mathieu

A2 - Johnstone, Scott

PB - Springer Protocols

CY - New York

ER -

Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction

Abstract

Keywords

Fingerprint

Projects

OZR2651: International Joint Research Group - Liver Connexin and Pannexin Research Group (LCPR)

EU457: CONNECT: Connexin and pannexin channels as drug targets and biomarkers in acute and chronic liver disease

IWT680: Characterisatin of connexin43 and pannexin1 as well as corresponding hemichannels as biomarkers and pharmacological targets in acute liver failure: a combined vitro-in vivo study

Activities

The role of connexins, pannexins and their channels in liver fibrosis

Cite this