Metabolomics is the comprehensive study of small-molecule metabolites. Obtaining a wide coverage of the metabolome is challenging because of the broad range of physicochemical properties of the small molecules. To study the compounds of interest spectroscopic (NMR), spectrometric (MS) and separation techniques (LC, GC, supercritical fluid chromatography, CE) are used. The choice for a given technique is influenced by the sample matrix, the concentration and properties of the metabolites, and the amount of sample. This review discusses the most commonly used analytical techniques for metabolomic studies, including their advantages, drawbacks and some applications.

Original languageEnglish
Pages (from-to)2297-2318
Number of pages22
Issue number24
Publication statusPublished - 17 Dec 2019


  • multiplatform approaches, separation techniques, spectrometric techniques, spectroscopic techniques, targeted metabolomics, untargeted metabolomics


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