Arabidopsis Casein Kinase 2 triggers stem cell exhaustion under Al toxicity and phosphate deficiency through activation of the DNA damage response pathway

Pengliang Wei, Manon Demulder, Pascale David, Thomas Eekhout, Kaoru O. Yoshiyama, Long Nguyen, Ilse Vercauteren, Dominique Eeckhout, Margot Galle, Paul Larsen, Geert De Jaeger, Dominique Audenaert, Thierry Desnod, Laurent Nussaume, Remy Loris, Lieven De Veylder

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)
26 Downloads (Pure)

Abstract

Aluminum (Al) toxicity and inorganic phosphate (Pi) limitation are widespread chronic abiotic and mutually enhancing stresses that profoundly affect crop yield. Both stresses cause a strong inhibition of root growth, resulting from a progressive exhaustion of the stem cell niche. Here, we report on a casein kinase 2 (CK2) inhibitor identified by its capability to maintain a functional root stem cell niche under Al toxic conditions. CK2 operates through phosphorylation of the cell cycle checkpoint activator SUPPRESSOR OF GAMMA RADIATION1 (SOG1), priming its activity under DNA-damaging conditions. In addition to yielding Al tolerance, CK2 and SOG1 inactivation prevents meristem exhaustion under Pi starvation, revealing the existence of a low Pi-induced cell cycle checkpoint that depends on the DNA damage activator ATAXIA-TELANGIECTASIA MUTATED. Overall, our data reveal an important physiological role for the plant DNA damage response pathway under agriculturally limiting growth conditions, opening new avenues to cope with Pi limitation.
Original languageEnglish
Pages (from-to)1361-1380
Number of pages20
JournalPlant Cell
Volume33
Issue number4
DOIs
Publication statusPublished - Apr 2021

Keywords

  • Casein kinase 2
  • SOG1
  • Aluminium tolerance

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