Biophysical characterization of the Plasmodium falciparum circumsporozoite protein’s N-terminal domain

Rob Geens; Jessica Stanisich; O Beyens; S. D'Hondt; J.M. Thiberge; A. Ryckebosch; A.  De Groot; Stefan Magez; Didier Vertommen; R. Amino; H. De Winter; A.N. Volkov; Peter Tompa; Y.G.J. Sterckx

doi:10.1002/pro.4852

Biophysical characterization of the Plasmodium falciparum circumsporozoite protein’s N-terminal domain

Rob Geens, Jessica Stanisich, O Beyens, S. D'Hondt, J.M. Thiberge, A. Ryckebosch, A. De Groot, Stefan Magez, Didier Vertommen, R. Amino, H. De Winter, A.N. Volkov, Peter Tompa, Y.G.J. Sterckx

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Abstract

The circumsporozoite protein (CSP) is the main surface antigen of the Plasmodium sporozoite (SPZ) and forms the basis of the currently only licensed anti-malarial vaccine (RTS,S/AS01). CSP uniformly coats the SPZ and plays a pivotal role in its immunobiology, in both the insect and the vertebrate hosts. Although CSP's N-terminal domain (CSP_N) has been reported to play an important role in multiple CSP functions, a thorough biophysical and structural characterization of CSP_N is currently lacking. Here, we present an alternative method for the recombinant production and purification of CSP_N from Plasmodium falciparum (PfCSP_N), which provides pure, high-quality protein preparations with high yields. Through an interdisciplinary approach combining in-solution experimental methods and in silico analyses, we provide strong evidence that PfCSP_N is an intrinsically disordered region displaying some degree of compaction.

Original language	English
Article number	e4852
Number of pages	17
Journal	Protein Science : A Publication of the Protein Society
Volume	33
Issue number	1
DOIs	https://doi.org/10.1002/pro.4852
Publication status	Published - Jan 2024

Bibliographical note

Keywords

Humans
Plasmodium falciparum/genetics
Malaria Vaccines/chemistry
Protozoan Proteins/genetics
Antimalarials
Malaria, Falciparum

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Geens, R., Stanisich, J., Beyens, O., D'Hondt, S., Thiberge, J. M., Ryckebosch, A., De Groot, A., Magez, S., Vertommen, D., Amino, R., De Winter, H., Volkov, A. N., Tompa, P., & Sterckx, Y. G. J. (2024). Biophysical characterization of the Plasmodium falciparum circumsporozoite protein’s N-terminal domain. Protein Science : A Publication of the Protein Society, 33(1), Article e4852. https://doi.org/10.1002/pro.4852

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title = "Biophysical characterization of the Plasmodium falciparum circumsporozoite protein{\textquoteright}s N-terminal domain",

abstract = "The circumsporozoite protein (CSP) is the main surface antigen of the Plasmodium sporozoite (SPZ) and forms the basis of the currently only licensed anti-malarial vaccine (RTS,S/AS01). CSP uniformly coats the SPZ and plays a pivotal role in its immunobiology, in both the insect and the vertebrate hosts. Although CSP's N-terminal domain (CSPN) has been reported to play an important role in multiple CSP functions, a thorough biophysical and structural characterization of CSPN is currently lacking. Here, we present an alternative method for the recombinant production and purification of CSPN from Plasmodium falciparum (PfCSPN), which provides pure, high-quality protein preparations with high yields. Through an interdisciplinary approach combining in-solution experimental methods and in silico analyses, we provide strong evidence that PfCSPN is an intrinsically disordered region displaying some degree of compaction.",

keywords = "Humans, Plasmodium falciparum/genetics, Malaria Vaccines/chemistry, Protozoan Proteins/genetics, Antimalarials, Malaria, Falciparum",

author = "Rob Geens and Jessica Stanisich and O Beyens and S. D'Hondt and J.M. Thiberge and A. Ryckebosch and {De Groot}, A. and Stefan Magez and Didier Vertommen and R. Amino and {De Winter}, H. and A.N. Volkov and Peter Tompa and Y.G.J. Sterckx",

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Geens, R, Stanisich, J, Beyens, O, D'Hondt, S, Thiberge, JM, Ryckebosch, A, De Groot, A, Magez, S, Vertommen, D, Amino, R, De Winter, H, Volkov, AN , Tompa, P & Sterckx, YGJ 2024, 'Biophysical characterization of the Plasmodium falciparum circumsporozoite protein’s N-terminal domain', Protein Science : A Publication of the Protein Society, vol. 33, no. 1, e4852. https://doi.org/10.1002/pro.4852

TY - JOUR

T1 - Biophysical characterization of the Plasmodium falciparum circumsporozoite protein’s N-terminal domain

AU - Geens, Rob

AU - Stanisich, Jessica

AU - Beyens, O

AU - D'Hondt, S.

AU - Thiberge, J.M.

AU - Ryckebosch, A.

AU - De Groot, A.

AU - Magez, Stefan

AU - Vertommen, Didier

AU - Amino, R.

AU - De Winter, H.

AU - Volkov, A.N.

AU - Tompa, Peter

AU - Sterckx, Y.G.J.

PY - 2024/1

Y1 - 2024/1

N2 - The circumsporozoite protein (CSP) is the main surface antigen of the Plasmodium sporozoite (SPZ) and forms the basis of the currently only licensed anti-malarial vaccine (RTS,S/AS01). CSP uniformly coats the SPZ and plays a pivotal role in its immunobiology, in both the insect and the vertebrate hosts. Although CSP's N-terminal domain (CSPN) has been reported to play an important role in multiple CSP functions, a thorough biophysical and structural characterization of CSPN is currently lacking. Here, we present an alternative method for the recombinant production and purification of CSPN from Plasmodium falciparum (PfCSPN), which provides pure, high-quality protein preparations with high yields. Through an interdisciplinary approach combining in-solution experimental methods and in silico analyses, we provide strong evidence that PfCSPN is an intrinsically disordered region displaying some degree of compaction.

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KW - Humans

KW - Plasmodium falciparum/genetics

KW - Malaria Vaccines/chemistry

KW - Protozoan Proteins/genetics

KW - Antimalarials

KW - Malaria, Falciparum

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U2 - 10.1002/pro.4852

DO - 10.1002/pro.4852

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SN - 0961-8368

VL - 33

JO - Protein Science : A Publication of the Protein Society

JF - Protein Science : A Publication of the Protein Society

IS - 1

M1 - e4852

ER -

Biophysical characterization of the Plasmodium falciparum circumsporozoite protein’s N-terminal domain

Abstract

Bibliographical note

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