TY - JOUR
T1 - Body composition and blood pressure of 6-year-old singletons born after Pre-implantation Genetic Testing (PGT): a matched cohort study
AU - Belva, Florence
AU - Roelants, M.
AU - Kluijfhout, Salome Adriana
AU - Winter, Christiane
AU - Desmyttere, Sonja
AU - Buysse, Andrea
AU - De Becker, Pascale
AU - De Rycke, Martine
AU - Verpoest, Willem
AU - Liebaers, Ingeborg
AU - Bonduelle, Mary-Louise
PY - 2018
Y1 - 2018
N2 - Summary answer: Blood pressure and anthropometric measurements
including body mass index (BMI) and indices of peripheral and central adiposity
were comparable between PGT and ICSI children.
What is known already: Although neonatal outcomes after PGT conception
have been found comparable to those after ICSI, few studies have reported on
the medical outcome at older ages. We previously found that at age 2, mean
body mass index tended to be lower in PGT children while the prevalence of
congenital anomalies was similar. Others reported that blood pressure and
anthropometrics did not differ in 4- and 9-year old children conceived after
IVF/ICSI with or without aneuploidy testing (PGT-A). However, outcomes in
PGT-A children cannot be extrapolated to PGT children given the dissimilar
indications and parental reproductive background.
Study design, size, duration: In this single-center study, singletons conceived
by PGT reaching between 5 and 6 years during the period May 2011 and June
2017 were matched as closely as possible for gender, age, maternal educational
level and birth order to peers born after ICSI. Biopsy of cleavage-stage embryos
was performed solely for the purpose of PGT (PGT-A cycles were excluded) and
all fresh embryo transfers in PGT and ICSI cycles were carried out at day 5.
Participants/materials, setting, methods: Anthropometrics (weight,
height, BMI, skinfold thickness, waist and mid-upper arm circumference) and
blood pressure readings of 87 singletons born after PGT, including PGT for
mongenic defects (PGT-M) and chromosomal structural rearrangements (PGTSR),
but not for aneuploidies (PGT-A), were compared with results of 87 peers
born after ICSI.
Main results and the role of chance: From the 124 eligible PGD families,
110 could be contacted, 23 declined and 87 (70.2%) children participated of
whom 39 (45%) were males. The reasons for declining were ‘no time’ and ‘living
too far from the hospital’. Neonatal characteristics, including birth weight
Standard Deviation Score (SDS) and gestational age and parental characteristics,
including maternal weight gain, smoking and alcohol consumption during pregnancy,
maternal age, current maternal and paternal BMI were comparable
between the groups (all p > 0.05). Mean age was 5.5 years in the PGT group and
5.6 years in the ICSI group (P = 0.2). All anthropometric measurements, including
BMI SDS, waist and mid-upper arm circumference SDS were comparable
between the PGT (-0.23; 0.27; 0.17 respectively) and ICSI (-0.29; 0.10; 0.11)
group (all P > 0.05). Furthermore, skin thickness derived indices of peripheral
and central adiposity were comparable (PGT: 14.7mm; 11.6mm and ICSI:
15.5mm; 11.5mm) as well as the calculated total body fat mass (PGT: 13.7%
and ICSI: 13.9%) (all P > 0.05). Finally, also blood pressure SDS were comparable
between the two groups (all P > 0.05). Results did not change taking neonatal
(birth weight SDS, gestational age, birth order) and parental (pre-pregnancy
maternal BMI, maternal educational level) characteristics into account.
Limitations, reasons for caution: The non-participating PGT children did
not differ from the participating group in terms of gender, birth order, gestational
age, birth weight or maternal educational level, which makes participation
bias less likely.
Wider implications of the findings: Our results point to no adverse effect
of embryo biopsy at cleavage-stage and fresh embryo transfer at day 5.
However, more and long-term follow-up studies are indicated as there are currently
no data available for PGT children born after trophectoderm biopsy and/
or transfer of warmed embryos after vitrification.
Trial registration number: not applicable.
Abstracts of the 34th Annual Meeting of the ESHRE, Barcelona, Spain 1 to 4 July 2018 i31
AB - Summary answer: Blood pressure and anthropometric measurements
including body mass index (BMI) and indices of peripheral and central adiposity
were comparable between PGT and ICSI children.
What is known already: Although neonatal outcomes after PGT conception
have been found comparable to those after ICSI, few studies have reported on
the medical outcome at older ages. We previously found that at age 2, mean
body mass index tended to be lower in PGT children while the prevalence of
congenital anomalies was similar. Others reported that blood pressure and
anthropometrics did not differ in 4- and 9-year old children conceived after
IVF/ICSI with or without aneuploidy testing (PGT-A). However, outcomes in
PGT-A children cannot be extrapolated to PGT children given the dissimilar
indications and parental reproductive background.
Study design, size, duration: In this single-center study, singletons conceived
by PGT reaching between 5 and 6 years during the period May 2011 and June
2017 were matched as closely as possible for gender, age, maternal educational
level and birth order to peers born after ICSI. Biopsy of cleavage-stage embryos
was performed solely for the purpose of PGT (PGT-A cycles were excluded) and
all fresh embryo transfers in PGT and ICSI cycles were carried out at day 5.
Participants/materials, setting, methods: Anthropometrics (weight,
height, BMI, skinfold thickness, waist and mid-upper arm circumference) and
blood pressure readings of 87 singletons born after PGT, including PGT for
mongenic defects (PGT-M) and chromosomal structural rearrangements (PGTSR),
but not for aneuploidies (PGT-A), were compared with results of 87 peers
born after ICSI.
Main results and the role of chance: From the 124 eligible PGD families,
110 could be contacted, 23 declined and 87 (70.2%) children participated of
whom 39 (45%) were males. The reasons for declining were ‘no time’ and ‘living
too far from the hospital’. Neonatal characteristics, including birth weight
Standard Deviation Score (SDS) and gestational age and parental characteristics,
including maternal weight gain, smoking and alcohol consumption during pregnancy,
maternal age, current maternal and paternal BMI were comparable
between the groups (all p > 0.05). Mean age was 5.5 years in the PGT group and
5.6 years in the ICSI group (P = 0.2). All anthropometric measurements, including
BMI SDS, waist and mid-upper arm circumference SDS were comparable
between the PGT (-0.23; 0.27; 0.17 respectively) and ICSI (-0.29; 0.10; 0.11)
group (all P > 0.05). Furthermore, skin thickness derived indices of peripheral
and central adiposity were comparable (PGT: 14.7mm; 11.6mm and ICSI:
15.5mm; 11.5mm) as well as the calculated total body fat mass (PGT: 13.7%
and ICSI: 13.9%) (all P > 0.05). Finally, also blood pressure SDS were comparable
between the two groups (all P > 0.05). Results did not change taking neonatal
(birth weight SDS, gestational age, birth order) and parental (pre-pregnancy
maternal BMI, maternal educational level) characteristics into account.
Limitations, reasons for caution: The non-participating PGT children did
not differ from the participating group in terms of gender, birth order, gestational
age, birth weight or maternal educational level, which makes participation
bias less likely.
Wider implications of the findings: Our results point to no adverse effect
of embryo biopsy at cleavage-stage and fresh embryo transfer at day 5.
However, more and long-term follow-up studies are indicated as there are currently
no data available for PGT children born after trophectoderm biopsy and/
or transfer of warmed embryos after vitrification.
Trial registration number: not applicable.
Abstracts of the 34th Annual Meeting of the ESHRE, Barcelona, Spain 1 to 4 July 2018 i31
M3 - Meeting abstract (Journal)
VL - 33
SP - 31
EP - 31
JO - Human Reproduction
JF - Human Reproduction
SN - 0268-1161
IS - Suppl
T2 - ESHRE 2018
Y2 - 2 July 2018 through 4 July 2018
ER -