Brain Perfusion Alterations Induced by Standalone and Combined Non-Invasive Brain Stimulation over the Dorsolateral Prefrontal Cortex

Lais Boralli Razza; Pedro Henrique Rodrigues da Silva; Geraldo F Busatto; Fábio Luis de Souza Duran; Juliana Pereira; Stefanie De Smet; Izio Klein; Tamires A Zanão; Matthias S Luethi; Chris Baeken; Marie-Anne Vanderhasselt; Carlos Alberto Buchpiguel; André Russowsky Brunoni

doi:10.3390/biomedicines10102410

Brain Perfusion Alterations Induced by Standalone and Combined Non-Invasive Brain Stimulation over the Dorsolateral Prefrontal Cortex

Lais Boralli Razza, Pedro Henrique Rodrigues da Silva, Geraldo F Busatto, Fábio Luis de Souza Duran, Juliana Pereira, Stefanie De Smet, Izio Klein, Tamires A Zanão, Matthias S Luethi, Chris Baeken, Marie-Anne Vanderhasselt, Carlos Alberto Buchpiguel, André Russowsky Brunoni

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Abstract

Non-invasive brain stimulation (NIBS) interventions are promising for the treatment of psychiatric disorders. Notwithstanding, the NIBS mechanisms of action over the dorsolateral prefrontal cortex (DLPFC), a hub that modulates affective and cognitive processes, have not been completely mapped. We aimed to investigate regional cerebral blood flow (rCBF) changes over the DLPFC and the subgenual anterior cingulate cortex (sgACC) of different NIBS protocols using Single-Photon Emission Computed Tomography (SPECT). A factorial, within-subjects, double-blinded study was performed. Twenty-three healthy subjects randomly underwent four sessions of NIBS applied once a week: transcranial direct current stimulation (tDCS), intermittent theta-burst stimulation (iTBS), combined tDCS + iTBS and placebo. The radiotracer 99m-Technetium-ethylene-cysteine-dimer was injected intravenously during the NIBS session, and SPECT neuroimages were acquired after the session. Results revealed that the combination of tDCS + iTBS increased right sgACC rCBF. Cathodal and anodal tDCS increased and decreased DLPFC rCBF, respectively, while iTBS showed no significant changes compared to the placebo. Our findings suggest that the combined protocol might optimize the activity in the right sgACC and encourage future trials with neuropsychiatric populations. Moreover, mechanistic studies to investigate the effects of tDCS and iTBS over the DLPFC are required.

Original language	English
Article number	2410
Number of pages	11
Journal	Biomedicines
Volume	10
Issue number	10
DOIs	https://doi.org/10.3390/biomedicines10102410
Publication status	Published - Oct 2022

Bibliographical note

Funding Information:
A.R.B. receives grants from the National Council for Scientific and Technological Development (PQ-1B), FAPESP (Grants: 2018/10861-7, 2019/06009-6) and Academy of Medical Sciences (Newton Advanced Fellowship Research, NAFR 12/1010). A.R.B. has a small equity of Flow™, whose devices were not used in the present study. The LIM-27 laboratory receives grants from the Associação Beneficente Alzira Denise Hertzog da Silva. L.B.R. was supported by FAPESP (Grant: 2019/07256-7). M.-A.V. received funding from the Research Foundation Flanders (FWO) and from the Ghent University (Grants: FWO.OPR.2019.0100.01 and BOFSTA2017002501, respectively). S.D.S. is supported by FWO (Grant: 11J7521N).

Publisher Copyright:
© 2022 by the authors.

Copyright:
Copyright 2022 Elsevier B.V., All rights reserved.

Keywords

Single-Photon Emission Computed Tomography (SPECT)
combined interventions
dorsolateral prefrontal cortex (DLPFC)
non-invasive brain stimulation (NIBS)
synergistic effect

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Razza, L. B., da Silva, P. H. R., Busatto, G. F., Duran, F. L. D. S., Pereira, J., De Smet, S., Klein, I., Zanão, T. A., Luethi, M. S., Baeken, C., Vanderhasselt, M.-A., Buchpiguel, C. A., & Brunoni, A. R. (2022). Brain Perfusion Alterations Induced by Standalone and Combined Non-Invasive Brain Stimulation over the Dorsolateral Prefrontal Cortex. Biomedicines, 10(10), Article 2410. https://doi.org/10.3390/biomedicines10102410

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title = "Brain Perfusion Alterations Induced by Standalone and Combined Non-Invasive Brain Stimulation over the Dorsolateral Prefrontal Cortex",

abstract = "Non-invasive brain stimulation (NIBS) interventions are promising for the treatment of psychiatric disorders. Notwithstanding, the NIBS mechanisms of action over the dorsolateral prefrontal cortex (DLPFC), a hub that modulates affective and cognitive processes, have not been completely mapped. We aimed to investigate regional cerebral blood flow (rCBF) changes over the DLPFC and the subgenual anterior cingulate cortex (sgACC) of different NIBS protocols using Single-Photon Emission Computed Tomography (SPECT). A factorial, within-subjects, double-blinded study was performed. Twenty-three healthy subjects randomly underwent four sessions of NIBS applied once a week: transcranial direct current stimulation (tDCS), intermittent theta-burst stimulation (iTBS), combined tDCS + iTBS and placebo. The radiotracer 99m-Technetium-ethylene-cysteine-dimer was injected intravenously during the NIBS session, and SPECT neuroimages were acquired after the session. Results revealed that the combination of tDCS + iTBS increased right sgACC rCBF. Cathodal and anodal tDCS increased and decreased DLPFC rCBF, respectively, while iTBS showed no significant changes compared to the placebo. Our findings suggest that the combined protocol might optimize the activity in the right sgACC and encourage future trials with neuropsychiatric populations. Moreover, mechanistic studies to investigate the effects of tDCS and iTBS over the DLPFC are required.",

keywords = "Single-Photon Emission Computed Tomography (SPECT), combined interventions, dorsolateral prefrontal cortex (DLPFC), non-invasive brain stimulation (NIBS), synergistic effect",

author = "Razza, {Lais Boralli} and {da Silva}, {Pedro Henrique Rodrigues} and Busatto, {Geraldo F} and Duran, {F{\'a}bio Luis de Souza} and Juliana Pereira and {De Smet}, Stefanie and Izio Klein and Zan{\~a}o, {Tamires A} and Luethi, {Matthias S} and Chris Baeken and Marie-Anne Vanderhasselt and Buchpiguel, {Carlos Alberto} and Brunoni, {Andr{\'e} Russowsky}",

note = "Funding Information: A.R.B. receives grants from the National Council for Scientific and Technological Development (PQ-1B), FAPESP (Grants: 2018/10861-7, 2019/06009-6) and Academy of Medical Sciences (Newton Advanced Fellowship Research, NAFR 12/1010). A.R.B. has a small equity of Flow{\texttrademark}, whose devices were not used in the present study. The LIM-27 laboratory receives grants from the Associa{\c c}{\~a}o Beneficente Alzira Denise Hertzog da Silva. L.B.R. was supported by FAPESP (Grant: 2019/07256-7). M.-A.V. received funding from the Research Foundation Flanders (FWO) and from the Ghent University (Grants: FWO.OPR.2019.0100.01 and BOFSTA2017002501, respectively). S.D.S. is supported by FWO (Grant: 11J7521N). Publisher Copyright: {\textcopyright} 2022 by the authors. Copyright: Copyright 2022 Elsevier B.V., All rights reserved.",

year = "2022",

month = oct,

doi = "10.3390/biomedicines10102410",

language = "English",

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Razza, LB, da Silva, PHR, Busatto, GF, Duran, FLDS, Pereira, J, De Smet, S, Klein, I, Zanão, TA, Luethi, MS, Baeken, C, Vanderhasselt, M-A, Buchpiguel, CA & Brunoni, AR 2022, 'Brain Perfusion Alterations Induced by Standalone and Combined Non-Invasive Brain Stimulation over the Dorsolateral Prefrontal Cortex', Biomedicines, vol. 10, no. 10, 2410. https://doi.org/10.3390/biomedicines10102410

TY - JOUR

T1 - Brain Perfusion Alterations Induced by Standalone and Combined Non-Invasive Brain Stimulation over the Dorsolateral Prefrontal Cortex

AU - Razza, Lais Boralli

AU - da Silva, Pedro Henrique Rodrigues

AU - Busatto, Geraldo F

AU - Duran, Fábio Luis de Souza

AU - Pereira, Juliana

AU - De Smet, Stefanie

AU - Klein, Izio

AU - Zanão, Tamires A

AU - Luethi, Matthias S

AU - Baeken, Chris

AU - Vanderhasselt, Marie-Anne

AU - Buchpiguel, Carlos Alberto

AU - Brunoni, André Russowsky

N1 - Funding Information: A.R.B. receives grants from the National Council for Scientific and Technological Development (PQ-1B), FAPESP (Grants: 2018/10861-7, 2019/06009-6) and Academy of Medical Sciences (Newton Advanced Fellowship Research, NAFR 12/1010). A.R.B. has a small equity of Flow™, whose devices were not used in the present study. The LIM-27 laboratory receives grants from the Associação Beneficente Alzira Denise Hertzog da Silva. L.B.R. was supported by FAPESP (Grant: 2019/07256-7). M.-A.V. received funding from the Research Foundation Flanders (FWO) and from the Ghent University (Grants: FWO.OPR.2019.0100.01 and BOFSTA2017002501, respectively). S.D.S. is supported by FWO (Grant: 11J7521N). Publisher Copyright: © 2022 by the authors. Copyright: Copyright 2022 Elsevier B.V., All rights reserved.

PY - 2022/10

Y1 - 2022/10

N2 - Non-invasive brain stimulation (NIBS) interventions are promising for the treatment of psychiatric disorders. Notwithstanding, the NIBS mechanisms of action over the dorsolateral prefrontal cortex (DLPFC), a hub that modulates affective and cognitive processes, have not been completely mapped. We aimed to investigate regional cerebral blood flow (rCBF) changes over the DLPFC and the subgenual anterior cingulate cortex (sgACC) of different NIBS protocols using Single-Photon Emission Computed Tomography (SPECT). A factorial, within-subjects, double-blinded study was performed. Twenty-three healthy subjects randomly underwent four sessions of NIBS applied once a week: transcranial direct current stimulation (tDCS), intermittent theta-burst stimulation (iTBS), combined tDCS + iTBS and placebo. The radiotracer 99m-Technetium-ethylene-cysteine-dimer was injected intravenously during the NIBS session, and SPECT neuroimages were acquired after the session. Results revealed that the combination of tDCS + iTBS increased right sgACC rCBF. Cathodal and anodal tDCS increased and decreased DLPFC rCBF, respectively, while iTBS showed no significant changes compared to the placebo. Our findings suggest that the combined protocol might optimize the activity in the right sgACC and encourage future trials with neuropsychiatric populations. Moreover, mechanistic studies to investigate the effects of tDCS and iTBS over the DLPFC are required.

AB - Non-invasive brain stimulation (NIBS) interventions are promising for the treatment of psychiatric disorders. Notwithstanding, the NIBS mechanisms of action over the dorsolateral prefrontal cortex (DLPFC), a hub that modulates affective and cognitive processes, have not been completely mapped. We aimed to investigate regional cerebral blood flow (rCBF) changes over the DLPFC and the subgenual anterior cingulate cortex (sgACC) of different NIBS protocols using Single-Photon Emission Computed Tomography (SPECT). A factorial, within-subjects, double-blinded study was performed. Twenty-three healthy subjects randomly underwent four sessions of NIBS applied once a week: transcranial direct current stimulation (tDCS), intermittent theta-burst stimulation (iTBS), combined tDCS + iTBS and placebo. The radiotracer 99m-Technetium-ethylene-cysteine-dimer was injected intravenously during the NIBS session, and SPECT neuroimages were acquired after the session. Results revealed that the combination of tDCS + iTBS increased right sgACC rCBF. Cathodal and anodal tDCS increased and decreased DLPFC rCBF, respectively, while iTBS showed no significant changes compared to the placebo. Our findings suggest that the combined protocol might optimize the activity in the right sgACC and encourage future trials with neuropsychiatric populations. Moreover, mechanistic studies to investigate the effects of tDCS and iTBS over the DLPFC are required.

KW - Single-Photon Emission Computed Tomography (SPECT)

KW - combined interventions

KW - dorsolateral prefrontal cortex (DLPFC)

KW - non-invasive brain stimulation (NIBS)

KW - synergistic effect

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U2 - 10.3390/biomedicines10102410

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VL - 10

JO - Biomedicines

JF - Biomedicines

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M1 - 2410

ER -

Brain Perfusion Alterations Induced by Standalone and Combined Non-Invasive Brain Stimulation over the Dorsolateral Prefrontal Cortex

Abstract

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SRP85: SRP-Onderzoekszwaartepunt: Translating the biology of cognitive function to improve diagnosis and treatment of brain disorders

SRP57: SRP-Onderzoekszwaartepunt: The Social and Cognitive Brain

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Brain Perfusion Alterations Induced by Standalone and Combined Non-Invasive Brain Stimulation over the Dorsolateral Prefrontal Cortex

Abstract

Bibliographical note

Keywords

Access to Document

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Projects

SRP85: SRP-Onderzoekszwaartepunt: Translating the biology of cognitive function to improve diagnosis and treatment of brain disorders

SRP57: SRP-Onderzoekszwaartepunt: The Social and Cognitive Brain

Cite this