Brytella acorum gen. nov., sp. nov., a novel acetic acid bacterium from sour beverages

Atena Sadat Sombolestani, Dries Bongaerts, Eliza Depoorter, Ilse Cleenwerck, Anneleen D. Wieme, Scott J. Britton, Stefan Weckx, Luc De Vuyst, Peter Vandamme

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Polyphasic taxonomic and comparative genomic analyses revealed that a series of lambic beer isolates including strain LMG 32668T and the kombucha isolate LMG 32879 represent a novel species among the acetic acid bacteria, with Acidomonas methanolica as the nearest phylogenomic neighbor with a valid name. Overall genomic relatedness indices and phylogenomic and physiological analyses revealed that this novel species was best classified in a novel genus for which we propose the name Brytella acorum gen. nov., sp. nov., with LMG 32668T(=CECT 30723T) as the type strain. The B. acorum genomes encode a complete but modified tricarboxylic acid cycle, and complete pentose phosphate, pyruvate oxidation and gluconeogenesis pathways. The absence of 6‐
phosphofructokinase which rendered the glycolysis pathway non‐functional, and an energy metabolism that included both aerobic respiration and oxidative fermentation are typical metabolic characteristics of acetic acid bacteria. Neither genome encodes nitrogen fixation or nitrate reduction genes, but both genomes encode genes for the biosynthesis of a broad range of amino acids. Antibiotic resistance genes or virulence factors are absent.
Original languageEnglish
Article number126440
Number of pages8
JournalSystematic and Applied Microbiology
Volume46
Issue number5
DOIs
Publication statusPublished - Sep 2023

Bibliographical note

Funding Information:
The BCCM/LMG Bacteria Collection is supported by the Federal Public Planning Service - Science Policy, Belgium. A. S. is indebted to the Special Research Council of Ghent University for a scholarship. The computational resources and services used in this work were provided by the VSC (Flemish Supercomputer Center), funded by the Research Foundation Flanders (FWO) and the Flemish Government – department EWI. L. D. V. acknowledges his financial support by the Research Council of the Vrije Universiteit Brussel (SRP and IOF/GEAR projects). D. B. is the recipient of a VUB doctoral fellowship.

Funding Information:
We thank the Oxford Genomics Centre at the Welcome Centre for Human Genetics (funded by Wellcome Trust grant reference 203141/Z/16/Z) for the generation and initial processing of the sequencing data. We thank Marjan De Wachter for the MALDI-TOF MS data acquisition.

Publisher Copyright:
© 2023 Elsevier GmbH

Keywords

  • Brytella
  • Acetobacteraceae
  • Acetic acid bacteria
  • Sour beverages
  • Kombucha
  • Lambic beer

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