Astrocytes seem to play a key role in demyelination resistance because, compared to other strains of mice, B6 display an enhanced astrocytic anti-viral response that might contribute to their ability to clear TMEV (Carpentier et al., Virology. 375 (1): 24-36, 2008). Nevertheless the mechanisms involved in acute seizures and epilepsy development have not been elucidated yet within this model.
Increased excitation due to altered glutamate transporters levels has been described in epilepsy (Meldrum et al., Epilepsy Res. 36: 189-204, 1999). The aim of our work was to unravel the involvement of glial glutamate transporters in the TMEV model of viral-induced epilepsy. Therefore, protein expression levels of GLAST and GLT1, two glutamate reuptake transporters, and xCT, the functional subunit of cystine/glutamate antiporter or system xc- which is the main source of extracellular glutamate in mouse hippocampus, were investigated by Western Blotting. Cortical (CRX), hippocampal (HP) and cerebellar (CER) samples were collected during the acute phase (5 days p.i.), the clearance phase (14 days p.i.) and the chronic phase (2 months p.i.) of B6 mice infected with DA strain of TMEV and compared with their mock-infected littermates.
Our findings show changes in glial glutamate transporters expression that may be involved in seizure generation and development of subsequent epilepsy. Further research is needed to elucidate whether increased levels of system xc- together with changes in glutamate re-uptake carriers lead to a rise in extracellular glutamate levels and hyperexcitability in viral-induced epilepsy.
|Title of host publication||11th meeting of the Belgian Society for Neuroscience, May 22, Mons, Belgium|
|Publication status||Published - 2015|
|Event||BSN 2015 - 11th Meeting of the Belgian Society for Neuroscience - University of Mons, Mons, Belgium|
Duration: 22 May 2015 → 22 May 2015
|Seminar||BSN 2015 - 11th Meeting of the Belgian Society for Neuroscience|
|Period||22/05/15 → 22/05/15|