TY - JOUR
T1 - Cholestasis differentially affects liver connexins.
AU - Cooreman, Axelle
AU - Van Campenhout, Raf
AU - Crespo Yanguas, Sara
AU - Gijbels, Eva
AU - Leroy, Kaat
AU - Pieters, Alanah
AU - Tabernilla Garcia, Andres
AU - Van Brantegem, Pieter
AU - Annaert, Pieter
AU - Cogliati, Bruno
AU - Vinken, Mathieu
PY - 2020/9/7
Y1 - 2020/9/7
N2 - Connexins are goal keepers of tissue homeostasis, including in liver. As a result, they are frequently involved in disease. The current study was set up to investigate the effects of cholestatic disease on the production of connexin26, connexin32 and connexin43 in liver. For this purpose, bile duct ligation, a well-known trigger of cholestatic liver injury, was applied to mice. In parallel, human hepatoma HepaRG cell cultures were exposed to cholestatic drugs and bile acids. Samples from both the in vivo and in vitro settings were subsequently subjected to assessment of mRNA and protein quantities as well as to in situ immunostaining. While the outcome of cholestasis on connexin26 and connexin43 varied among experimental settings, a more generalized repressing effect was seen for connexin32. This has also been observed in many other liver pathologies and could suggest a role for connexin32 as a robust biomarker of liver disease and toxicity.
AB - Connexins are goal keepers of tissue homeostasis, including in liver. As a result, they are frequently involved in disease. The current study was set up to investigate the effects of cholestatic disease on the production of connexin26, connexin32 and connexin43 in liver. For this purpose, bile duct ligation, a well-known trigger of cholestatic liver injury, was applied to mice. In parallel, human hepatoma HepaRG cell cultures were exposed to cholestatic drugs and bile acids. Samples from both the in vivo and in vitro settings were subsequently subjected to assessment of mRNA and protein quantities as well as to in situ immunostaining. While the outcome of cholestasis on connexin26 and connexin43 varied among experimental settings, a more generalized repressing effect was seen for connexin32. This has also been observed in many other liver pathologies and could suggest a role for connexin32 as a robust biomarker of liver disease and toxicity.
KW - connexin
KW - liver
KW - cholestasis
U2 - 10.3390/ijms21186534
DO - 10.3390/ijms21186534
M3 - Article
VL - 21
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 18
M1 - 6534
ER -