Circadian serum progesterone variations on the day of frozen embryo transfer in artificially prepared cycles

Sara Loreti; Caroline Roelens; Panagiotis Drakopoulos; Neelke De Munck; Herman Tournaye; Shari Mackens; Christophe Blockeel

doi:https://doi.org/10.1016/j.rbmo.2023.103601

Circadian serum progesterone variations on the day of frozen embryo transfer in artificially prepared cycles

Sara Loreti, Caroline Roelens, Panagiotis Drakopoulos, Neelke De Munck, Herman Tournaye, Shari Mackens, Christophe Blockeel

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Research question: What is the intra-day variation of serum progesterone related to vaginal progesterone administration on the day of frozen embryo transfer (FET) in an artificial cycle? Design: A prospective cohort study was conducted including 22 patients undergoing a single blastocyst artificial cycle (AC)–FET from August to December 2022. Endometrial preparation was achieved by administering oestradiol valerate (2 mg three times daily) and consecutively micronized vaginal progesterone (MVP; 400 mg twice daily). A blastocyst FET was performed on the 6th day of MVP administration. Serum progesterone concentrations were measured on the day of transfer at 08:00, 12:00, 16:00 and 20:00 hours. The first and last blood samples were collected just before MVP was administered. Results: The mean age and body mass index of the study population were 33.95 ± 3.98 years and 23.10 ± 1.95 kg/m2. The mean P-values at 08:00, 12:00, 16:00 and 20:00 hours were 11.72 ± 4.99, 13.59 ± 6.33, 10.23 ± 3.81 and 9.28 ± 3.09 ng/ml, respectively. A significant decline, of 2.41 ng/ml (95% confidence interval 0.81–4.00), was found between the first and last progesterone measurements. Conclusion: A statistically significant intra-day variation of serum progesterone concentrations on the day of FET in artificially prepared cycles was observed. This highlights the importance of a standardized procedure for the timing of progesterone measurement on the day of AC–FET. Of note, the study results are applicable only to women using MVP for luteal phase support; therefore it is necessary to confirm its validity in comparison with the different existing administration routes of progesterone.

Original language	English
Article number	103601
Number of pages	8
Journal	Reproductive BioMedicine Online
Volume	48
Issue number	1
DOIs	https://doi.org/10.1016/j.rbmo.2023.103601 https://doi.org/10.1016/j.rbmo.2023.103601
Publication status	Published - Jan 2024

Bibliographical note

Funding Information:
The authors thank the women who voluntarily participated and the staff of Brussels IVF, Brussels, Belgium. C.B. S.M. C.R. and S.L. are responsible for the concept and study design and for manuscript writing. S.L. performed the data collection and drafted the manuscript. P.D. performed the statistical and data analyses. H.T. and N.D.M. revised the manuscript critically for important intellectual content. All authors contributed to the interpretation, discussion and editing of the manuscript. All the authors approved the final version.

Publisher Copyright:
© 2023 Reproductive Healthcare Ltd.

Keywords

Artificial cycle
Circadian variation
Frozen embryo transfer
Serum progesterone

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@article{25e7084c0f5e4e6283e56da0ff3a1501,

title = "Circadian serum progesterone variations on the day of frozen embryo transfer in artificially prepared cycles",

abstract = "Research question: What is the intra-day variation of serum progesterone related to vaginal progesterone administration on the day of frozen embryo transfer (FET) in an artificial cycle? Design: A prospective cohort study was conducted including 22 patients undergoing a single blastocyst artificial cycle (AC)–FET from August to December 2022. Endometrial preparation was achieved by administering oestradiol valerate (2 mg three times daily) and consecutively micronized vaginal progesterone (MVP; 400 mg twice daily). A blastocyst FET was performed on the 6th day of MVP administration. Serum progesterone concentrations were measured on the day of transfer at 08:00, 12:00, 16:00 and 20:00 hours. The first and last blood samples were collected just before MVP was administered. Results: The mean age and body mass index of the study population were 33.95 ± 3.98 years and 23.10 ± 1.95 kg/m2. The mean P-values at 08:00, 12:00, 16:00 and 20:00 hours were 11.72 ± 4.99, 13.59 ± 6.33, 10.23 ± 3.81 and 9.28 ± 3.09 ng/ml, respectively. A significant decline, of 2.41 ng/ml (95% confidence interval 0.81–4.00), was found between the first and last progesterone measurements. Conclusion: A statistically significant intra-day variation of serum progesterone concentrations on the day of FET in artificially prepared cycles was observed. This highlights the importance of a standardized procedure for the timing of progesterone measurement on the day of AC–FET. Of note, the study results are applicable only to women using MVP for luteal phase support; therefore it is necessary to confirm its validity in comparison with the different existing administration routes of progesterone.",

keywords = "Artificial cycle, Circadian variation, Frozen embryo transfer, Serum progesterone",

author = "Sara Loreti and Caroline Roelens and Panagiotis Drakopoulos and {De Munck}, Neelke and Herman Tournaye and Shari Mackens and Christophe Blockeel",

note = "Funding Information: The authors thank the women who voluntarily participated and the staff of Brussels IVF, Brussels, Belgium. C.B. S.M. C.R. and S.L. are responsible for the concept and study design and for manuscript writing. S.L. performed the data collection and drafted the manuscript. P.D. performed the statistical and data analyses. H.T. and N.D.M. revised the manuscript critically for important intellectual content. All authors contributed to the interpretation, discussion and editing of the manuscript. All the authors approved the final version. Publisher Copyright: {\textcopyright} 2023 Reproductive Healthcare Ltd.",

year = "2024",

month = jan,

doi = "https://doi.org/10.1016/j.rbmo.2023.103601",

language = "English",

volume = "48",

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Circadian serum progesterone variations on the day of frozen embryo transfer in artificially prepared cycles. / Loreti, Sara ; Roelens, Caroline ; Drakopoulos, Panagiotis ; De Munck, Neelke ; Tournaye, Herman ; Mackens, Shari ; Blockeel, Christophe.

In: Reproductive BioMedicine Online, Vol. 48, No. 1, 103601, 01.2024.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Circadian serum progesterone variations on the day of frozen embryo transfer in artificially prepared cycles

AU - Loreti, Sara

AU - Roelens, Caroline

AU - Drakopoulos, Panagiotis

AU - De Munck, Neelke

AU - Tournaye, Herman

AU - Mackens, Shari

AU - Blockeel, Christophe

N1 - Funding Information: The authors thank the women who voluntarily participated and the staff of Brussels IVF, Brussels, Belgium. C.B. S.M. C.R. and S.L. are responsible for the concept and study design and for manuscript writing. S.L. performed the data collection and drafted the manuscript. P.D. performed the statistical and data analyses. H.T. and N.D.M. revised the manuscript critically for important intellectual content. All authors contributed to the interpretation, discussion and editing of the manuscript. All the authors approved the final version. Publisher Copyright: © 2023 Reproductive Healthcare Ltd.

PY - 2024/1

Y1 - 2024/1

N2 - Research question: What is the intra-day variation of serum progesterone related to vaginal progesterone administration on the day of frozen embryo transfer (FET) in an artificial cycle? Design: A prospective cohort study was conducted including 22 patients undergoing a single blastocyst artificial cycle (AC)–FET from August to December 2022. Endometrial preparation was achieved by administering oestradiol valerate (2 mg three times daily) and consecutively micronized vaginal progesterone (MVP; 400 mg twice daily). A blastocyst FET was performed on the 6th day of MVP administration. Serum progesterone concentrations were measured on the day of transfer at 08:00, 12:00, 16:00 and 20:00 hours. The first and last blood samples were collected just before MVP was administered. Results: The mean age and body mass index of the study population were 33.95 ± 3.98 years and 23.10 ± 1.95 kg/m2. The mean P-values at 08:00, 12:00, 16:00 and 20:00 hours were 11.72 ± 4.99, 13.59 ± 6.33, 10.23 ± 3.81 and 9.28 ± 3.09 ng/ml, respectively. A significant decline, of 2.41 ng/ml (95% confidence interval 0.81–4.00), was found between the first and last progesterone measurements. Conclusion: A statistically significant intra-day variation of serum progesterone concentrations on the day of FET in artificially prepared cycles was observed. This highlights the importance of a standardized procedure for the timing of progesterone measurement on the day of AC–FET. Of note, the study results are applicable only to women using MVP for luteal phase support; therefore it is necessary to confirm its validity in comparison with the different existing administration routes of progesterone.

AB - Research question: What is the intra-day variation of serum progesterone related to vaginal progesterone administration on the day of frozen embryo transfer (FET) in an artificial cycle? Design: A prospective cohort study was conducted including 22 patients undergoing a single blastocyst artificial cycle (AC)–FET from August to December 2022. Endometrial preparation was achieved by administering oestradiol valerate (2 mg three times daily) and consecutively micronized vaginal progesterone (MVP; 400 mg twice daily). A blastocyst FET was performed on the 6th day of MVP administration. Serum progesterone concentrations were measured on the day of transfer at 08:00, 12:00, 16:00 and 20:00 hours. The first and last blood samples were collected just before MVP was administered. Results: The mean age and body mass index of the study population were 33.95 ± 3.98 years and 23.10 ± 1.95 kg/m2. The mean P-values at 08:00, 12:00, 16:00 and 20:00 hours were 11.72 ± 4.99, 13.59 ± 6.33, 10.23 ± 3.81 and 9.28 ± 3.09 ng/ml, respectively. A significant decline, of 2.41 ng/ml (95% confidence interval 0.81–4.00), was found between the first and last progesterone measurements. Conclusion: A statistically significant intra-day variation of serum progesterone concentrations on the day of FET in artificially prepared cycles was observed. This highlights the importance of a standardized procedure for the timing of progesterone measurement on the day of AC–FET. Of note, the study results are applicable only to women using MVP for luteal phase support; therefore it is necessary to confirm its validity in comparison with the different existing administration routes of progesterone.

KW - Artificial cycle

KW - Circadian variation

KW - Frozen embryo transfer

KW - Serum progesterone

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DO - https://doi.org/10.1016/j.rbmo.2023.103601

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VL - 48

JO - Reproductive BioMedicine Online

JF - Reproductive BioMedicine Online

SN - 1472-6483

IS - 1

M1 - 103601

ER -

Circadian serum progesterone variations on the day of frozen embryo transfer in artificially prepared cycles

Abstract

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Keywords

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