Circumventing toxicity: different methods to produce Vibrio cholerae ParE2 without killing the host cell

Bacterial toxins inhibit their host cell by disrupting vital processes. So does the ParE2 toxin of V. cholerae, a protein known to target Gyrase. This topoisomerase is present in bacteria but not in eukaryotic cells, making it an interesting antibiotic-target. Understanding how ParE2 acts on Gyrase may be a starting point to develop novel classes of antibiotics. However, free toxin has to be obtained. This study shows different cloning/ expression strategies that are used to overcome ParE2 toxicity