Co-transplantation of mesenchymal stem cells for efficient spermatogonial stem cell transplantation

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Spermatogonial stem cell transplantation (SSCT) is a promising fertility preservation technique for pre-pubertal cancer patients. However, transplanting only SSCs results in low colonization efficiency in mice. This might be due to a deficient SSC niche. We hypothesize that mesenchymal stem cells (MSCs) may contribute to the SSC niche and improve transplantation efficiency. GFP+ SSCs and RFP+ MSCs were transplanted to busulfan and cadmium chloride treated sterile mice. Four groups of ten mice were identified. The first group received only SSCs (SSCT), the second group received only MSCs (MSCT), the third group received both SSCs and MSCs (MS-SSCT) and the fourth group received SSCs and TGFß1-treated MSCs (MSi-SSCT). After three months, donor-derived spermatogenesis (GFP+) was found in 21.4% of successfully injected
testes after SSCT, 0% after MSCT, in 12.5% after MS-SSCT and in 50% after MSi-
SSCT. After MSCT and MS-SSCT, some transplanted MSCs started to express MVH (germ cell marker) and SOX9 (Sertoli cell marker). MSCs in the MSi-SSCT group only showed MVH expression. MSCs were also found to have migrated to the visceral organs after MSCT and MS-SSCT but not after MSi-SSCT. Thus, co-transplantation of TGFß1 induced MSCs might improve the efficiency of SSC transplantation.
Original languageEnglish
Number of pages1
Publication statusPublished - 8 May 2017
Event11th International Congress of Andrology - Copenhagen, Denmark
Duration: 6 May 20179 May 2017


Conference11th International Congress of Andrology


  • Spermatogonial stem cells
  • Mesenchymal stem cells
  • Transplantation


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