Abstract The P31T mutant of Staphylococcus aureus thioredoxin crystallizes spontaneously in space group P212121 with unit cell parameters a = 41.7 Å, b = 49.5 Å, c=55.6 Å. The crystals diffract to 2.2 Å resolution. Isomorphous crystals of wild type thioredoxin as well as of other point mutants only grow when seeded with the P31T mutant. Our results suggest seeding as a valuable tool complementing surface engineering for proteins that are hard to crystallize.
|Number of pages||4|
|Journal||Acta Crystallogr F Struct Biol Commun|
|Publication status||Published - Dec 2006|
- macromolecular crystallography